Detailed information of TA system TA238967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5499822..5500347
• Replicon: chromosome
• Accession: NZ_CP093856
• Organism: Klebsiella pneumoniae strain Bio30

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: W8EAA6
• Locus tag: MOQ79_RS27190
• Protein ID: WP_008322213.1
• Coordinates: 5500042..5500347 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A6C0NE25
• Locus tag: MOQ79_RS27185
• Protein ID: WP_032934863.1
• Coordinates: 5499822..5500040 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ79_RS27165 (5494860)	5494860..5495207	+	348	Protein_5350	transposase	-
MOQ79_RS27170 (5495463)	5495463..5496338	+	876	WP_000239590.1	extended-spectrum class A beta-lactamase CTX-M-15	-
MOQ79_RS27175 (5496385)	5496385..5496729	-	345	Protein_5352	WbuC family cupin fold metalloprotein	-
MOQ79_RS27180 (5496743)	5496743..5498974	+	2232	Protein_5353	Tn3-like element Tn3 family transposase	-
MOQ79_RS27185 (5499822)	5499822..5500040	+	219	WP_032934863.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
MOQ79_RS27190 (5500042)	5500042..5500347	+	306	WP_008322213.1	type II toxin-antitoxin system toxin CcdB	Toxin
MOQ79_RS27195 (5500349)	5500349..5500663	+	315	WP_008322211.1	hypothetical protein	-
MOQ79_RS27200 (5500653)	5500653..5501444	+	792	WP_008322208.1	site-specific integrase	-
MOQ79_RS27205 (5501643)	5501643..5502983	-	1341	Protein_5358	GntP family transporter	-
MOQ79_RS27210 (5503024)	5503024..5503122	-	99	WP_242809244.1	type IV conjugative transfer system pilin TraA	-
MOQ79_RS27215 (5503190)	5503190..5503390	-	201	WP_046664192.1	TraY domain-containing protein	-
MOQ79_RS27220 (5503476)	5503476..5504177	-	702	WP_117046017.1	hypothetical protein	-
MOQ79_RS27225 (5504407)	5504407..5504799	-	393	WP_004194114.1	conjugal transfer relaxosome DNA-binding protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	blaCTX-M-15 / aac(3)-IIa / aac(6')-Ib-cr / blaOXA-1	manB	5482631..5576855	94224
	-	flank	IS/Tn	blaCTX-M-15	-	5495463..5499022	3559

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11450.23 Da
• Isoelectric Point: 5.8211

>T238967 WP_008322213.1 NZ_CP093856:5500042-5500347 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Nucleotide

• Length: 306 bp

>T238967 NZ_LR890547:c4736891-4736607 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8297.11 Da
• Isoelectric Point: 4.4013

>AT238967 WP_032934863.1 NZ_CP093856:5499822-5500040 [Klebsiella pneumoniae]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Nucleotide

• Length: 219 bp

>AT238967 NZ_LR890547:c4737123-4736881 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A6C0NE27

Antitoxin

• AlphaFold DB: A0A6C0NE25