Detailed information of TA system TA238966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 5493917..5494720
• Replicon: chromosome
• Accession: NZ_CP093856
• Organism: Klebsiella pneumoniae strain Bio30

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: W8E895
• Locus tag: MOQ79_RS27160
• Protein ID: WP_016479963.1
• Coordinates: 5494190..5494720 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W8E6Q5
• Locus tag: MOQ79_RS27155
• Protein ID: WP_022652312.1
• Coordinates: 5493917..5494186 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ79_RS27140 (5489276)	5489276..5490304	-	1029	WP_004177874.1	UDP-N-acetylmuramate dehydrogenase	-
MOQ79_RS27145 (5491037)	5491037..5491776	+	740	Protein_5346	site-specific integrase	-
MOQ79_RS27150 (5492072)	5492072..5493061	-	990	WP_022652311.1	RepB family plasmid replication initiator protein	-
MOQ79_RS27155 (5493917)	5493917..5494186	+	270	WP_022652312.1	DUF1778 domain-containing protein	Antitoxin
MOQ79_RS27160 (5494190)	5494190..5494720	+	531	WP_016479963.1	GNAT family N-acetyltransferase	Toxin
MOQ79_RS27165 (5494860)	5494860..5495207	+	348	Protein_5350	transposase	-
MOQ79_RS27170 (5495463)	5495463..5496338	+	876	WP_000239590.1	extended-spectrum class A beta-lactamase CTX-M-15	-
MOQ79_RS27175 (5496385)	5496385..5496729	-	345	Protein_5352	WbuC family cupin fold metalloprotein	-
MOQ79_RS27180 (5496743)	5496743..5498974	+	2232	Protein_5353	Tn3-like element Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	blaCTX-M-15 / aac(3)-IIa / aac(6')-Ib-cr / blaOXA-1	manB	5482631..5576855	94224
	-	flank	IS/Tn	blaCTX-M-15	-	5495463..5499022	3559

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20350.24 Da
• Isoelectric Point: 6.4907

>T238966 WP_016479963.1 NZ_CP093856:5494190-5494720 [Klebsiella pneumoniae]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Nucleotide

• Length: 531 bp

>T238966 NZ_LR890547:c4635047-4634514 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10269.70 Da
• Isoelectric Point: 5.7252

>AT238966 WP_022652312.1 NZ_CP093856:5493917-5494186 [Klebsiella pneumoniae]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Nucleotide

• Length: 270 bp

>AT238966 NZ_LR890547:c4635324-4635058 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A6C0L7Q2

Antitoxin

• AlphaFold DB: A0A2Z3XDS5