Detailed information of TA system TA238965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5375787..5376312
• Replicon: chromosome
• Accession: NZ_CP093856
• Organism: Klebsiella pneumoniae strain Bio30

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: MOQ79_RS26405
• Protein ID: WP_114502755.1
• Coordinates: 5375787..5376092 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: MOQ79_RS26410
• Protein ID: WP_004197642.1
• Coordinates: 5376094..5376312 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ79_RS26375 (5371497)	5371497..5372123	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
MOQ79_RS26380 (5372120)	5372120..5372422	+	303	WP_004197636.1	hypothetical protein	-
MOQ79_RS26385 (5372862)	5372862..5373656	-	795	WP_048991183.1	site-specific integrase	-
MOQ79_RS26390 (5373854)	5373854..5374870	-	1017	WP_242809239.1	hypothetical protein	-
MOQ79_RS26395 (5374867)	5374867..5375190	-	324	WP_004197641.1	hypothetical protein	-
MOQ79_RS26400 (5375217)	5375217..5375618	-	402	WP_072199347.1	hypothetical protein	-
MOQ79_RS26405 (5375787)	5375787..5376092	-	306	WP_114502755.1	type II toxin-antitoxin system toxin CcdB	Toxin
MOQ79_RS26410 (5376094)	5376094..5376312	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
MOQ79_RS26415 (5376908)	5376908..5377177	+	270	WP_073558205.1	hypothetical protein	-
MOQ79_RS26420 (5377165)	5377165..5377740	+	576	WP_073558204.1	hypothetical protein	-
MOQ79_RS26425 (5377771)	5377771..5378265	+	495	WP_117264656.1	DNA-binding protein	-
MOQ79_RS26430 (5378309)	5378309..5378677	+	369	WP_117046016.1	hypothetical protein	-
MOQ79_RS26435 (5378711)	5378711..5378914	+	204	WP_004213596.1	HHA domain-containing protein	-
MOQ79_RS26440 (5378928)	5378928..5379158	+	231	WP_049118762.1	hypothetical protein	-
MOQ79_RS26445 (5379275)	5379275..5379529	+	255	WP_025862265.1	hypothetical protein	-
MOQ79_RS26450 (5379665)	5379665..5380441	-	777	WP_110133704.1	DUF2971 domain-containing protein	-
MOQ79_RS26455 (5380682)	5380682..5381005	-	324	WP_110133703.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11631.30 Da
• Isoelectric Point: 6.4661

>T238965 WP_114502755.1 NZ_CP093856:c5376092-5375787 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASTRLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T238965 NZ_LR890547:4015169-4015387 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT238965 WP_004197642.1 NZ_CP093856:c5376312-5376094 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT238965 NZ_LR890547:4014768-4015142 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTTTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG