Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5015408..5016033 Replicon chromosome
Accession NZ_CP093856
Organism Klebsiella pneumoniae strain Bio30

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MOQ79_RS24500 Protein ID WP_002882817.1
Coordinates 5015650..5016033 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MOQ79_RS24495 Protein ID WP_004150355.1
Coordinates 5015408..5015650 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOQ79_RS24470 (5011399) 5011399..5011998 + 600 WP_004151865.1 glucose-1-phosphatase -
MOQ79_RS24475 (5011992) 5011992..5012852 + 861 WP_002882807.1 virulence factor BrkB family protein -
MOQ79_RS24480 (5012849) 5012849..5013286 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MOQ79_RS24485 (5013331) 5013331..5014272 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MOQ79_RS24490 (5014286) 5014286..5015203 - 918 WP_040238240.1 alpha/beta hydrolase -
MOQ79_RS24495 (5015408) 5015408..5015650 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MOQ79_RS24500 (5015650) 5015650..5016033 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MOQ79_RS24505 (5016207) 5016207..5017136 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MOQ79_RS24510 (5017133) 5017133..5017768 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MOQ79_RS24515 (5017765) 5017765..5018667 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T238963 WP_002882817.1 NZ_CP093856:5015650-5016033 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T238963 NZ_LR890547:1932262-1932364 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT238963 WP_004150355.1 NZ_CP093856:5015408-5015650 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT238963 NZ_LR890547:c1932371-1932226 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References