Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3249286..3250096 Replicon chromosome
Accession NZ_CP093856
Organism Klebsiella pneumoniae strain Bio30

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag MOQ79_RS15790 Protein ID WP_004178461.1
Coordinates 3249286..3249819 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MOQ79_RS15795 Protein ID WP_002887278.1
Coordinates 3249830..3250096 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOQ79_RS15770 (3244393) 3244393..3245748 + 1356 WP_004180947.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
MOQ79_RS15775 (3245790) 3245790..3246113 + 324 WP_002914095.1 YfiM family lipoprotein -
MOQ79_RS15780 (3246117) 3246117..3247415 - 1299 WP_004144368.1 MFS transporter -
MOQ79_RS15785 (3248117) 3248117..3249238 + 1122 WP_009309849.1 cupin domain-containing protein -
MOQ79_RS15790 (3249286) 3249286..3249819 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
MOQ79_RS15795 (3249830) 3249830..3250096 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MOQ79_RS15800 (3250199) 3250199..3251632 - 1434 WP_023280821.1 Cu(+)/Ag(+) sensor histidine kinase -
MOQ79_RS15805 (3251622) 3251622..3252305 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MOQ79_RS15810 (3252478) 3252478..3253863 + 1386 WP_040238382.1 efflux transporter outer membrane subunit -
MOQ79_RS15815 (3253881) 3253881..3254225 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T238959 WP_004178461.1 NZ_CP093856:c3249819-3249286 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T238959 NZ_LR890547:328921-329289 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT238959 WP_002887278.1 NZ_CP093856:c3250096-3249830 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT238959 NZ_LR890547:328703-328924 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References