Detailed information of TA system TA238944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 11310..12061
• Replicon: plasmid pBio45
• Accession: NZ_CP093855
• Organism: Klebsiella pneumoniae strain Bio45

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: MOQ73_RS28135
• Protein ID: WP_048333033.1
• Coordinates: 11579..12061 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: MOQ73_RS28130
• Protein ID: WP_004902250.1
• Coordinates: 11310..11588 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ73_RS28095 (MOQ73_28095)	6949..7374	-	426	WP_000522996.1	organomercurial transporter MerC	-
MOQ73_RS28100 (MOQ73_28100)	7402..7677	-	276	WP_043907009.1	mercury resistance system periplasmic binding protein MerP	-
MOQ73_RS28105 (MOQ73_28105)	7693..8058	-	366	WP_004200999.1	mercuric ion transporter MerT	-
MOQ73_RS28110 (MOQ73_28110)	8130..8585	+	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
MOQ73_RS28115 (MOQ73_28115)	9238..9696	+	459	WP_014386535.1	hypothetical protein	-
MOQ73_RS28120 (MOQ73_28120)	10527..10868	+	342	WP_032437747.1	hypothetical protein	-
MOQ73_RS28125 (MOQ73_28125)	10976..11188	+	213	WP_016946198.1	hypothetical protein	-
MOQ73_RS28130 (MOQ73_28130)	11310..11588	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
MOQ73_RS28135 (MOQ73_28135)	11579..12061	+	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
MOQ73_RS28140 (MOQ73_28140)	13096..13635	+	540	WP_004902239.1	hypothetical protein	-
MOQ73_RS28145 (MOQ73_28145)	13740..14132	+	393	WP_032442757.1	hypothetical protein	-
MOQ73_RS28150 (MOQ73_28150)	14233..14988	+	756	WP_004902235.1	DUF2971 domain-containing protein	-
MOQ73_RS28155 (MOQ73_28155)	15015..15662	+	648	WP_014386537.1	EcsC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / blaOXA-232 / blaTEM-1A / dfrA14 / tet(D) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15	rmpA / iutA / iucD / iucC / iucB / iucA	1..337042	337042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T238944 WP_048333033.1 NZ_CP093855:11579-12061 [Klebsiella pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T238944 NZ_LR890542:325054-325422 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGTGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT238944 WP_004902250.1 NZ_CP093855:11310-11588 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT238944 NZ_LR890542:324836-325057 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3