Detailed information of TA system TA238942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 5607886..5608696
• Replicon: chromosome
• Accession: NZ_CP093854
• Organism: Klebsiella pneumoniae strain Bio45

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A060VJ83
• Locus tag: MOQ73_RS27265
• Protein ID: WP_004178461.1
• Coordinates: 5608163..5608696 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: MOQ73_RS27260
• Protein ID: WP_002887278.1
• Coordinates: 5607886..5608152 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ73_RS27240 (5603758)	5603758..5604102	-	345	WP_004197273.1	cation efflux system protein CusF	-
MOQ73_RS27245 (5604120)	5604120..5605505	-	1386	WP_004178457.1	efflux transporter outer membrane subunit	-
MOQ73_RS27250 (5605677)	5605677..5606360	+	684	WP_002887273.1	copper response regulator transcription factor CusR	-
MOQ73_RS27255 (5606350)	5606350..5607783	+	1434	WP_004178459.1	Cu(+)/Ag(+) sensor histidine kinase	-
MOQ73_RS27260 (5607886)	5607886..5608152	+	267	WP_002887278.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
MOQ73_RS27265 (5608163)	5608163..5608696	+	534	WP_004178461.1	type II toxin-antitoxin system toxin KacT	Toxin
MOQ73_RS27270 (5608744)	5608744..5609865	-	1122	WP_002887282.1	cupin domain-containing protein	-
MOQ73_RS27275 (5610176)	5610176..5610673	+	498	WP_001083725.1	trimethoprim-resistant dihydrofolate reductase DfrA12	-
MOQ73_RS27280 (5610785)	5610785..5611075	+	291	WP_001336345.1	DUF1010 domain-containing protein	-
MOQ73_RS27285 (5611081)	5611081..5611919	+	839	Protein_5384	AadA family aminoglycoside 3''-O-nucleotidyltransferase	-
MOQ73_RS27290 (5612036)	5612036..5612383	+	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-
MOQ73_RS27295 (5612377)	5612377..5613216	+	840	WP_000259031.1	sulfonamide-resistant dihydropteroate synthase Sul1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19810.65 Da
• Isoelectric Point: 5.2614

>T238942 WP_004178461.1 NZ_CP093854:5608163-5608696 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T238942 NZ_LR890537:136035-136451 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT238942 WP_002887278.1 NZ_CP093854:5607886-5608152 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT238942 NZ_LR890537:135808-136038 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A060VJ83

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1