Detailed information of TA system TA023894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 4377412..4377943
• Replicon: chromosome
• Accession: NC_012808
• Organism: Methylobacterium extorquens AM1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MEXAM1_RS20200
• Protein ID: WP_003605878.1
• Coordinates: 4377662..4377943 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C5B2A4
• Locus tag: MEXAM1_RS20195
• Protein ID: WP_003605879.1
• Coordinates: 4377412..4377675 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEXAM1_RS20170	4372557..4373528	+	972	WP_003606981.1	quinone oxidoreductase	-
MEXAM1_RS20175	4373612..4374238	+	627	WP_003606982.1	bifunctional nicotinamidase/pyrazinamidase	-
MEXAM1_RS20180	4374249..4375106	-	858	WP_015857326.1	aminotransferase class IV	-
MEXAM1_RS20185	4375093..4375722	-	630	WP_003607382.1	aminodeoxychorismate/anthranilate synthase component II	-
MEXAM1_RS20190	4375719..4377119	-	1401	WP_015857327.1	aminodeoxychorismate synthase component I	-
MEXAM1_RS20195	4377412..4377675	+	264	WP_003605879.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MEXAM1_RS20200	4377662..4377943	+	282	WP_003605878.1	type II toxin-antitoxin system YafQ family toxin	Toxin
MEXAM1_RS20205	4378060..4379406	+	1347	WP_003605876.1	30S ribosomal protein S12 methylthiotransferase RimO	-
MEXAM1_RS20210	4379503..4379688	+	186	WP_003605874.1	hypothetical protein	-
MEXAM1_RS20215	4379695..4380096	+	402	WP_003605873.1	DoxX family protein	-
MEXAM1_RS20220	4380185..4380376	+	192	WP_003605871.1	hypothetical protein	-
MEXAM1_RS20225	4380462..4381283	-	822	WP_015857328.1	nucleoside triphosphate pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-83)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10664.24 Da
• Isoelectric Point: 10.2159

>T23894 WP_003605878.1 NC_012808:4377662-4377943 [Methylorubrum extorquens AM1]
MRTIERTTQFKRDYKREMKGRHRASLQADLTAVLTELAADRPLAARLRDHALTGNWADHRDCHVKPDLVLIYRLAGTETL
QLVRLGSHAELGF

Nucleotide

• Length: 282 bp

>T23894 NC_012808:4377662-4377943 [Methylorubrum extorquens AM1]
ATGCGGACGATTGAGCGAACCACGCAGTTCAAGCGTGACTATAAGCGGGAGATGAAGGGCAGGCACCGGGCGAGCCTTCA
AGCCGACCTGACGGCGGTGCTGACCGAGTTGGCCGCGGATCGCCCCCTGGCGGCGCGCCTGCGCGACCACGCCCTGACCG
GCAACTGGGCCGACCATCGCGACTGCCACGTGAAGCCGGATCTGGTGCTGATCTATCGGCTTGCAGGAACGGAAACGCTC
CAATTGGTGCGCCTCGGCTCGCATGCCGAACTGGGCTTCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9356.81 Da
• Isoelectric Point: 4.4230

>AT23894 WP_003605879.1 NC_012808:4377412-4377675 [Methylorubrum extorquens AM1]
MPTKSLIQARIDADTKEEATAVLKAMGLTVSDAVRMMLVRVAREKALPFEPLVPNETTIAAIREARSGNLPGFTSVEALM
ADLDADD

Nucleotide

• Length: 264 bp

>AT23894 NC_012808:4377412-4377675 [Methylorubrum extorquens AM1]
ATGCCGACCAAGTCGCTGATCCAGGCGCGGATCGATGCCGACACCAAGGAAGAGGCCACAGCCGTTCTCAAGGCCATGGG
GCTGACCGTCTCCGATGCGGTTCGCATGATGCTCGTGCGCGTCGCGCGCGAGAAGGCGCTTCCCTTCGAGCCTCTGGTTC
CCAACGAAACGACCATCGCAGCCATCCGCGAGGCGCGGTCCGGTAACCTTCCGGGCTTCACCAGCGTCGAGGCGCTGATG
GCCGATCTCGATGCGGACGATTGA

Structures

Antitoxin

• AlphaFold DB: C5B2A4