Detailed information of TA system TA238929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 4307559..4308229
• Replicon: chromosome
• Accession: NZ_CP093854
• Organism: Klebsiella pneumoniae strain Bio45

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: MOQ73_RS20580
• Protein ID: WP_004213072.1
• Coordinates: 4307786..4308229 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: MOQ73_RS20575
• Protein ID: WP_004213073.1
• Coordinates: 4307559..4307789 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ73_RS20550 (4303782)	4303782..4304681	-	900	WP_004225022.1	nucleotide-binding protein	-
MOQ73_RS20555 (4304671)	4304671..4304961	-	291	WP_004213078.1	hypothetical protein	-
MOQ73_RS20560 (4305313)	4305313..4305519	+	207	WP_004213077.1	hypothetical protein	-
MOQ73_RS20565 (4305509)	4305509..4305802	-	294	WP_004213076.1	hypothetical protein	-
MOQ73_RS20570 (4305818)	4305818..4306951	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
MOQ73_RS20575 (4307559)	4307559..4307789	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MOQ73_RS20580 (4307786)	4307786..4308229	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
MOQ73_RS20585 (4308378)	4308378..4308629	+	252	WP_227640036.1	hypothetical protein	-
MOQ73_RS20590 (4308652)	4308652..4308956	-	305	Protein_4053	transposase	-
MOQ73_RS20595 (4309373)	4309373..4310007	+	635	Protein_4054	mucoid phenotype regulator RmpA2	-
MOQ73_RS20600 (4310526)	4310526..4310929	-	404	Protein_4055	GAF domain-containing protein	-
MOQ73_RS20605 (4311020)	4311020..4311940	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
MOQ73_RS20610 (4311989)	4311989..4312492	-	504	WP_004145311.1	DM13 domain-containing protein	-
MOQ73_RS20615 (4312543)	4312543..4312809	-	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	rmpA / iutA / iucD / iucC / iucB / iucA	4141614..4392505	250891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T238929 WP_004213072.1 NZ_CP093854:4307786-4308229 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T238929 NZ_LR890536:c4420562-4420353 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT238929 WP_004213073.1 NZ_CP093854:4307559-4307789 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT238929 NZ_LR890536:c4420356-4420054 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620