Detailed information of TA system TA238928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 4259370..4259892
• Replicon: chromosome
• Accession: NZ_CP093854
• Organism: Klebsiella pneumoniae strain Bio45

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A0C7KG00
• Locus tag: MOQ73_RS20305
• Protein ID: WP_038991638.1
• Coordinates: 4259370..4259654 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: MOQ73_RS20310
• Protein ID: WP_004181777.1
• Coordinates: 4259644..4259892 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ73_RS20270 (4255007)	4255007..4255351	+	345	WP_014386518.1	hypothetical protein	-
MOQ73_RS20275 (4255348)	4255348..4255719	+	372	WP_044816264.1	hypothetical protein	-
MOQ73_RS20280 (4255764)	4255764..4256951	-	1188	WP_043907031.1	RNA-guided endonuclease TnpB family protein	-
MOQ73_RS20285 (4256987)	4256987..4257415	+	429	WP_077256229.1	IS200/IS605 family transposase	-
MOQ73_RS20290 (4257494)	4257494..4257718	+	225	Protein_3993	transposase	-
MOQ73_RS20295 (4257990)	4257990..4259216	-	1227	WP_040120327.1	RNA-guided endonuclease TnpB family protein	-
MOQ73_RS20300 (4259252)	4259252..4259353	+	102	Protein_3995	IS200/IS605 family transposase	-
MOQ73_RS20305 (4259370)	4259370..4259654	-	285	WP_038991638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MOQ73_RS20310 (4259644)	4259644..4259892	-	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MOQ73_RS20315 (4260183)	4260183..4261982	+	1800	WP_043907032.1	ATP-dependent helicase	-
MOQ73_RS20320 (4262316)	4262316..4263302	+	987	WP_040120328.1	hypothetical protein	-
MOQ73_RS20325 (4263447)	4263447..4263800	+	354	WP_004181774.1	hypothetical protein	-
MOQ73_RS20330 (4263862)	4263862..4264683	+	822	WP_004181772.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	rmpA / iutA / iucD / iucC / iucB / iucA	4141614..4392505	250891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10993.73 Da
• Isoelectric Point: 10.6516

>T238928 WP_038991638.1 NZ_CP093854:c4259654-4259370 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Nucleotide

• Length: 285 bp

>T238928 NZ_LR890536:c4269691-4269377 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCACACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT238928 WP_004181777.1 NZ_CP093854:c4259892-4259644 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT238928 NZ_LR890536:c4269927-4269694 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A0C7KG00

Antitoxin

• AlphaFold DB: A0A2J4R0U8