Detailed information of TA system TA238920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 76031..76701
• Replicon: plasmid pBio73
• Accession: NZ_CP093853
• Organism: Klebsiella pneumoniae strain Bio73

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: MOQ71_RS28425
• Protein ID: WP_004213072.1
• Coordinates: 76031..76474 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: MOQ71_RS28430
• Protein ID: WP_004213073.1
• Coordinates: 76471..76701 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ71_RS28390 (MOQ71_28390)	71442..71717	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
MOQ71_RS28395 (MOQ71_28395)	71768..72271	+	504	WP_004145311.1	DM13 domain-containing protein	-
MOQ71_RS28400 (MOQ71_28400)	72320..73240	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
MOQ71_RS28405 (MOQ71_28405)	73331..73734	+	404	Protein_75	GAF domain-containing protein	-
MOQ71_RS28410 (MOQ71_28410)	74253..74887	-	635	Protein_76	mucoid phenotype regulator RmpA2	-
MOQ71_RS28415 (MOQ71_28415)	75304..75608	+	305	Protein_77	transposase	-
MOQ71_RS28420 (MOQ71_28420)	75631..75882	-	252	WP_227640036.1	hypothetical protein	-
MOQ71_RS28425 (MOQ71_28425)	76031..76474	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
MOQ71_RS28430 (MOQ71_28430)	76471..76701	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MOQ71_RS28435 (MOQ71_28435)	77309..78442	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
MOQ71_RS28440 (MOQ71_28440)	78458..78751	+	294	WP_004213076.1	hypothetical protein	-
MOQ71_RS28445 (MOQ71_28445)	78741..78947	-	207	WP_004213077.1	hypothetical protein	-
MOQ71_RS28450 (MOQ71_28450)	79299..79589	+	291	WP_004213078.1	hypothetical protein	-
MOQ71_RS28455 (MOQ71_28455)	79579..80478	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / blaTEM-1A / blaOXA-232 / dfrA14 / tet(D) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15	iucA / iucB / iucC / iucD / iutA / rmpA	1..332171	332171

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T238920 WP_004213072.1 NZ_CP093853:c76474-76031 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T238920 NZ_LR890536:c2694360-2694184 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTGAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT238920 WP_004213073.1 NZ_CP093853:c76701-76471 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT238920 NZ_LR890536:c2694138-2693722 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAACAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620