Detailed information of TA system TA023892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 250181..250763
• Replicon: chromosome
• Accession: NC_012808
• Organism: Methylobacterium extorquens AM1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: C5B351
• Locus tag: MEXAM1_RS01095
• Protein ID: WP_003600758.1
• Coordinates: 250488..250763 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: H1KK02
• Locus tag: MEXAM1_RS01090
• Protein ID: WP_003600760.1
• Coordinates: 250181..250498 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEXAM1_RS01075	245453..246427	-	975	WP_003600763.1	aliphatic sulfonate ABC transporter substrate-binding protein	-
MEXAM1_RS01080	246587..249373	+	2787	WP_003600762.1	[protein-PII] uridylyltransferase	-
MEXAM1_RS01085	249465..250073	+	609	WP_003600761.1	nucleotide exchange factor GrpE	-
MEXAM1_RS01090	250181..250498	-	318	WP_003600760.1	BrnA antitoxin family protein	Antitoxin
MEXAM1_RS01095	250488..250763	-	276	WP_003600758.1	BrnT family toxin	Toxin
MEXAM1_RS01100	250857..251714	-	858	WP_003600757.1	prephenate dehydratase	-
MEXAM1_RS01105	251731..252474	-	744	WP_003600755.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
MEXAM1_RS32395	252782..253699	+	918	WP_003600753.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-84)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-100)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10095.62 Da
• Isoelectric Point: 8.6014

>T23892 WP_003600758.1 NC_012808:c250763-250488 [Methylorubrum extorquens AM1]
MLIVWDEPKRLTNLQKHGLDFADFEAGFDFETALVAVAQPSATGSARMKIIGEFDGQTVVAAIIAPLGREAISLISLRRA
SRSERRLYYAR

Nucleotide

• Length: 276 bp

>T23892 NC_012808:c250763-250488 [Methylorubrum extorquens AM1]
ATGTTGATTGTCTGGGACGAGCCCAAGCGCCTGACCAACCTGCAAAAGCACGGTCTCGACTTCGCGGATTTCGAGGCGGG
CTTCGACTTCGAGACCGCTCTTGTCGCGGTTGCCCAACCGAGCGCCACTGGCTCAGCCCGAATGAAGATCATCGGCGAGT
TCGACGGACAGACGGTGGTGGCAGCGATCATCGCTCCCTTGGGACGGGAAGCGATCTCGCTCATCAGCCTGCGCCGAGCC
AGCCGGAGCGAACGGAGGCTCTACTATGCCCGCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11641.04 Da
• Isoelectric Point: 5.1832

>AT23892 WP_003600760.1 NC_012808:c250498-250181 [Methylorubrum extorquens AM1]
MPADKPDRRPTTTSEEAGIQAGIAADPDNPEWTEADFRSAVPFEQAFPVLAEKLRRTRGPQSAPTKQLVSLRLDQDVLLR
FRATGPGWQSRMNEALRRAAADLPD

Nucleotide

• Length: 318 bp

>AT23892 NC_012808:c250498-250181 [Methylorubrum extorquens AM1]
ATGCCCGCTGACAAGCCCGATCGGAGACCGACCACGACCTCGGAGGAGGCCGGGATCCAAGCCGGCATCGCGGCCGATCC
CGACAATCCGGAATGGACCGAAGCGGACTTTCGAAGCGCCGTGCCCTTCGAACAGGCCTTTCCCGTCCTCGCAGAGAAAC
TGCGCCGCACCCGCGGCCCCCAAAGCGCTCCGACCAAACAGCTCGTCAGCTTGAGGCTCGATCAGGACGTCCTGCTTCGT
TTCAGGGCGACCGGTCCGGGCTGGCAGTCTCGGATGAACGAAGCCCTGCGCCGGGCCGCGGCCGATCTTCCCGACTGA

Structures

Toxin

• AlphaFold DB: C5B351

Antitoxin

• AlphaFold DB: H1KK02