Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 1899..2809 Replicon plasmid pBio73
Accession NZ_CP093853
Organism Klebsiella pneumoniae strain Bio73

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag MOQ71_RS28045 Protein ID WP_004181896.1
Coordinates 2339..2809 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag MOQ71_RS28040 Protein ID WP_004181895.1
Coordinates 1899..2342 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOQ71_RS28030 (MOQ71_28030) 105..527 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
MOQ71_RS28035 (MOQ71_28035) 527..1798 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
MOQ71_RS28040 (MOQ71_28040) 1899..2342 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
MOQ71_RS28045 (MOQ71_28045) 2339..2809 + 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
MOQ71_RS28050 (MOQ71_28050) 2919..3179 - 261 WP_004026352.1 hypothetical protein -
MOQ71_RS28055 (MOQ71_28055) 3750..4931 - 1182 WP_004883380.1 IS481 family transposase -
MOQ71_RS28060 (MOQ71_28060) 5003..5161 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
MOQ71_RS28065 (MOQ71_28065) 5764..6738 + 975 WP_043907013.1 hypothetical protein -
MOQ71_RS28070 (MOQ71_28070) 6936..7313 - 378 WP_004181901.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / blaTEM-1A / blaOXA-232 / dfrA14 / tet(D) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 iucA / iucB / iucC / iucD / iutA / rmpA 1..332171 332171
- flank IS/Tn - - 3750..4931 1181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T238919 WP_004181896.1 NZ_CP093853:2339-2809 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T238919 NZ_LR890536:2254120-2254223 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT238919 WP_004181895.1 NZ_CP093853:1899-2342 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT238919 NZ_LR890536:c2254261-2253990 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References