Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 5570658..5571468 Replicon chromosome
Accession NZ_CP093852
Organism Klebsiella pneumoniae strain Bio73

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag MOQ71_RS27060 Protein ID WP_004178461.1
Coordinates 5570658..5571191 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MOQ71_RS27065 Protein ID WP_002887278.1
Coordinates 5571202..5571468 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOQ71_RS27030 (5566163) 5566163..5566801 - 639 WP_004210261.1 VWA domain-containing protein -
MOQ71_RS27035 (5567281) 5567281..5567739 - 459 WP_004026591.1 ribbon-helix-helix protein, CopG family -
MOQ71_RS27040 (5567742) 5567742..5568713 - 972 WP_231538241.1 hypothetical protein -
MOQ71_RS27045 (5568858) 5568858..5568965 - 108 Protein_5338 DUF4236 domain-containing protein -
MOQ71_RS27050 (5568976) 5568976..5569191 - 216 Protein_5339 HpcH/HpaI aldolase/citrate lyase family protein -
MOQ71_RS27055 (5569489) 5569489..5570610 + 1122 WP_002887282.1 cupin domain-containing protein -
MOQ71_RS27060 (5570658) 5570658..5571191 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
MOQ71_RS27065 (5571202) 5571202..5571468 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MOQ71_RS27070 (5571571) 5571571..5573004 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
MOQ71_RS27075 (5572994) 5572994..5573677 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MOQ71_RS27080 (5573849) 5573849..5575234 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
MOQ71_RS27085 (5575252) 5575252..5575596 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T238917 WP_004178461.1 NZ_CP093852:c5571191-5570658 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T238917 NZ_LR890536:2046080-2046331 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT238917 WP_002887278.1 NZ_CP093852:c5571468-5571202 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT238917 NZ_LR890536:2046328-2046582 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References