Detailed information of TA system TA238916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 5555981..5556732
• Replicon: chromosome
• Accession: NZ_CP093852
• Organism: Klebsiella pneumoniae strain Bio73

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: MOQ71_RS26980
• Protein ID: WP_048333033.1
• Coordinates: 5556250..5556732 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: MOQ71_RS26975
• Protein ID: WP_004902250.1
• Coordinates: 5555981..5556259 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ71_RS26940 (5551620)	5551620..5552045	-	426	WP_000522996.1	organomercurial transporter MerC	-
MOQ71_RS26945 (5552073)	5552073..5552348	-	276	WP_043907009.1	mercury resistance system periplasmic binding protein MerP	-
MOQ71_RS26950 (5552364)	5552364..5552729	-	366	WP_004200999.1	mercuric ion transporter MerT	-
MOQ71_RS26955 (5552801)	5552801..5553256	+	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
MOQ71_RS26960 (5553909)	5553909..5554367	+	459	WP_014386535.1	hypothetical protein	-
MOQ71_RS26965 (5555198)	5555198..5555539	+	342	WP_032437747.1	hypothetical protein	-
MOQ71_RS26970 (5555647)	5555647..5555859	+	213	WP_016946198.1	hypothetical protein	-
MOQ71_RS26975 (5555981)	5555981..5556259	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
MOQ71_RS26980 (5556250)	5556250..5556732	+	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
MOQ71_RS26985 (5557767)	5557767..5558306	+	540	WP_004902239.1	hypothetical protein	-
MOQ71_RS26990 (5558411)	5558411..5558803	+	393	WP_032442757.1	hypothetical protein	-
MOQ71_RS26995 (5558904)	5558904..5559659	+	756	WP_004902235.1	DUF2971 domain-containing protein	-
MOQ71_RS27000 (5559686)	5559686..5560333	+	648	WP_014386537.1	EcsC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5546268..5568965	22697

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T238916 WP_048333033.1 NZ_CP093852:5556250-5556732 [Klebsiella pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T238916 NZ_LR890536:1211029-1211364 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT238916 WP_004902250.1 NZ_CP093852:5555981-5556259 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT238916 NZ_LR890536:1210781-1211029 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCAGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGAAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3