Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4358763..4359388 Replicon chromosome
Accession NZ_CP093852
Organism Klebsiella pneumoniae strain Bio73

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MOQ71_RS20880 Protein ID WP_002882817.1
Coordinates 4359005..4359388 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MOQ71_RS20875 Protein ID WP_004150355.1
Coordinates 4358763..4359005 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOQ71_RS20850 (4354754) 4354754..4355353 + 600 WP_004151865.1 glucose-1-phosphatase -
MOQ71_RS20855 (4355347) 4355347..4356207 + 861 WP_004146232.1 virulence factor BrkB family protein -
MOQ71_RS20860 (4356204) 4356204..4356641 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MOQ71_RS20865 (4356686) 4356686..4357627 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MOQ71_RS20870 (4357641) 4357641..4358558 - 918 WP_004181612.1 alpha/beta hydrolase -
MOQ71_RS20875 (4358763) 4358763..4359005 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MOQ71_RS20880 (4359005) 4359005..4359388 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MOQ71_RS20885 (4359562) 4359562..4360491 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MOQ71_RS20890 (4360488) 4360488..4361123 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MOQ71_RS20895 (4361120) 4361120..4362022 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T238905 WP_002882817.1 NZ_CP093852:4359005-4359388 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T238905 NZ_LR890528:c86192-85710 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT238905 WP_004150355.1 NZ_CP093852:4358763-4359005 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT238905 NZ_LR890528:c86446-86180 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References