Detailed information of TA system TA238904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 4265330..4266000
• Replicon: chromosome
• Accession: NZ_CP093852
• Organism: Klebsiella pneumoniae strain Bio73

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: MOQ71_RS20410
• Protein ID: WP_004213072.1
• Coordinates: 4265557..4266000 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: MOQ71_RS20405
• Protein ID: WP_004213073.1
• Coordinates: 4265330..4265560 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ71_RS20380 (4261553)	4261553..4262452	-	900	WP_004225022.1	nucleotide-binding protein	-
MOQ71_RS20385 (4262442)	4262442..4262732	-	291	WP_004213078.1	hypothetical protein	-
MOQ71_RS20390 (4263084)	4263084..4263290	+	207	WP_004213077.1	hypothetical protein	-
MOQ71_RS20395 (4263280)	4263280..4263573	-	294	WP_004213076.1	hypothetical protein	-
MOQ71_RS20400 (4263589)	4263589..4264722	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
MOQ71_RS20405 (4265330)	4265330..4265560	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MOQ71_RS20410 (4265557)	4265557..4266000	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
MOQ71_RS20415 (4266149)	4266149..4266400	+	252	WP_227640036.1	hypothetical protein	-
MOQ71_RS20420 (4266423)	4266423..4266727	-	305	Protein_4021	transposase	-
MOQ71_RS20425 (4267144)	4267144..4267778	+	635	Protein_4022	mucoid phenotype regulator RmpA2	-
MOQ71_RS20430 (4268297)	4268297..4268700	-	404	Protein_4023	GAF domain-containing protein	-
MOQ71_RS20435 (4268791)	4268791..4269711	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
MOQ71_RS20440 (4269760)	4269760..4270263	-	504	WP_004145311.1	DM13 domain-containing protein	-
MOQ71_RS20445 (4270314)	4270314..4270580	-	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	galU / rmpA / iutA / iucD / iucC / iucB / iucA	4109532..4327922	218390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T238904 WP_004213072.1 NZ_CP093852:4265557-4266000 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T238904 NZ_LR890528:c65782-65366 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT238904 WP_004213073.1 NZ_CP093852:4265330-4265560 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT238904 NZ_LR890528:c66009-65779 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGAGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620