Detailed information of TA system TA238898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 701242..701861
• Replicon: chromosome
• Accession: NZ_CP093852
• Organism: Klebsiella pneumoniae strain Bio73

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: MOQ71_RS03335
• Protein ID: WP_002892050.1
• Coordinates: 701242..701460 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: J2DPF6
• Locus tag: MOQ71_RS03340
• Protein ID: WP_002892066.1
• Coordinates: 701487..701861 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ71_RS03290 (696643)	696643..697008	-	366	WP_043906948.1	hypothetical protein	-
MOQ71_RS03295 (696965)	696965..697204	-	240	WP_032443530.1	hypothetical protein	-
MOQ71_RS03300 (697289)	697289..697552	+	264	WP_002892011.1	type B 50S ribosomal protein L31	-
MOQ71_RS03305 (697552)	697552..697692	+	141	WP_002892018.1	type B 50S ribosomal protein L36	-
MOQ71_RS03310 (697689)	697689..698387	-	699	WP_004178762.1	GNAT family protein	-
MOQ71_RS03315 (698488)	698488..699939	+	1452	WP_002892023.1	PLP-dependent aminotransferase family protein	-
MOQ71_RS03320 (699914)	699914..700384	-	471	WP_002892026.1	YlaC family protein	-
MOQ71_RS03325 (700405)	700405..700545	+	141	WP_004147370.1	hypothetical protein	-
MOQ71_RS03330 (700517)	700517..701083	-	567	WP_002892030.1	maltose O-acetyltransferase	-
MOQ71_RS03335 (701242)	701242..701460	-	219	WP_002892050.1	HHA domain-containing protein	Toxin
MOQ71_RS03340 (701487)	701487..701861	-	375	WP_002892066.1	Hha toxicity modulator TomB	Antitoxin
MOQ71_RS03345 (702347)	702347..705493	-	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
MOQ71_RS03350 (705516)	705516..706709	-	1194	WP_004177236.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	acrB / acrA	522546..737297	214751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T238898 WP_002892050.1 NZ_CP093852:c701460-701242 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T238898 NZ_LR890527:3848039-3848257 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14368.06 Da
• Isoelectric Point: 4.8989

>AT238898 WP_002892066.1 NZ_CP093852:c701861-701487 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT238898 NZ_LR890527:3847638-3848012 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2

Antitoxin

• AlphaFold DB: A0A0H3GJ93