Detailed information of TA system TA238896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 4741..5252
• Replicon: plasmid unnamed3
• Accession: NZ_CP093551
• Organism: Escherichia coli strain YL03

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W0NIW1
• Locus tag: MPJ80_RS25555
• Protein ID: WP_020956903.1
• Coordinates: 4741..5016 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MPJ80_RS25560
• Protein ID: WP_020956904.1
• Coordinates: 5028..5252 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPJ80_RS25535 (MPJ80_25485)	964..1521	+	558	WP_001217881.1	recombinase family protein	-
MPJ80_RS25540 (MPJ80_25490)	1644..2624	+	981	WP_013213985.1	IS481-like element ISKpn27 family transposase	-
MPJ80_RS25545 (MPJ80_25495)	2900..3781	+	882	WP_004199234.1	carbapenem-hydrolyzing class A beta-lactamase KPC-2	-
MPJ80_RS25550 (MPJ80_25500)	4453..4719	-	267	WP_020956902.1	hypothetical protein	-
MPJ80_RS25555 (MPJ80_25505)	4741..5016	-	276	WP_020956903.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
MPJ80_RS25560 (MPJ80_25510)	5028..5252	-	225	WP_020956904.1	DUF6290 family protein	Antitoxin
MPJ80_RS25565 (MPJ80_25515)	5556..5804	-	249	WP_020956905.1	hypothetical protein	-
MPJ80_RS25570 (MPJ80_25520)	5862..6074	-	213	WP_020956906.1	hypothetical protein	-
MPJ80_RS25575 (MPJ80_25525)	6458..6739	-	282	WP_088912116.1	hypothetical protein	-
MPJ80_RS25580 (MPJ80_25530)	6744..6965	-	222	WP_020956907.1	helix-turn-helix domain-containing protein	-
MPJ80_RS25585 (MPJ80_25535)	6962..7207	-	246	WP_020956908.1	hypothetical protein	-
MPJ80_RS25590 (MPJ80_25540)	7204..7746	-	543	WP_020956909.1	hypothetical protein	-
MPJ80_RS25595 (MPJ80_25545)	7837..8361	-	525	WP_020956910.1	DnaJ domain-containing protein	-
MPJ80_RS25600 (MPJ80_25550)	8469..8828	+	360	WP_020956911.1	hypothetical protein	-
MPJ80_RS25605 (MPJ80_25555)	9184..10044	+	861	WP_000027057.1	broad-spectrum class A beta-lactamase TEM-1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2 / blaTEM-1B	-	1..53307	53307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-88)

Antitoxin

• Protein of unknown function DUF6290 (6-72)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10922.82 Da
• Isoelectric Point: 10.6500

>T238896 WP_020956903.1 NZ_CP093551:c5016-4741 [Escherichia coli]
MTRYRTEYTKRFVKEFKKIDTVHQRVIQKYIEAHINNALNPRLTGKILKGDKAEFIRWRIGDYRLIATIEDDKLLVIALS
VGHRKDVYKKF

Nucleotide

• Length: 276 bp

>T238896 NZ_LR890527:c2594221-2592899 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGATAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGTTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGCGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8535.52 Da
• Isoelectric Point: 4.3313

>AT238896 WP_020956904.1 NZ_CP093551:c5252-5028 [Escherichia coli]
MNKNMTKAIRLNDEDFNLFESYANAKGITFSELCKSAVREKIEDELDLQCANESYADYLSDKTTISHDELFKSM

Nucleotide

• Length: 225 bp

>AT238896 NZ_LR890527:c2594487-2594221 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACTCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A384ZT31