Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3783631..3784128 Replicon chromosome
Accession NZ_CP093548
Organism Escherichia coli strain YL03

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MPJ80_RS18305 Protein ID WP_001173343.1
Coordinates 3783955..3784128 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MPJ80_RS18300 Protein ID WP_000398619.1
Coordinates 3783631..3783927 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPJ80_RS18280 (3780360) 3780360..3781079 - 720 WP_000611288.1 two-component system response regulator DcuR -
MPJ80_RS18285 (3781076) 3781076..3782707 - 1632 WP_001216477.1 sensor histidine kinase -
MPJ80_RS18290 (3782888) 3782888..3783118 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
MPJ80_RS18295 (3783130) 3783130..3783402 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
MPJ80_RS18300 (3783631) 3783631..3783927 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MPJ80_RS18305 (3783955) 3783955..3784128 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MPJ80_RS18310 (3784247) 3784247..3785764 - 1518 WP_000003806.1 lysine--tRNA ligase -
MPJ80_RS18315 (3786001) 3786001..3787458 - 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T238879 WP_001173343.1 NZ_CP093548:3783955-3784128 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T238879 NZ_LR890519:69663-69812 [Klebsiella pneumoniae]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT238879 WP_000398619.1 NZ_CP093548:3783631-3783927 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT238879 NZ_LR890519:c69618-69559 [Klebsiella pneumoniae]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References