Detailed information of TA system TA238852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 207920..208704
• Replicon: chromosome
• Accession: NZ_CP093548
• Organism: Escherichia coli strain YL03

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: MPJ80_RS01035
• Protein ID: WP_000613626.1
• Coordinates: 207920..208414 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: MPJ80_RS01040
• Protein ID: WP_001110447.1
• Coordinates: 208411..208704 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPJ80_RS01015 (203113)	203113..204210	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
MPJ80_RS01020 (204210)	204210..205151	+	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
MPJ80_RS01025 (205217)	205217..206860	+	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
MPJ80_RS01030 (206872)	206872..207825	+	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
MPJ80_RS01035 (207920)	207920..208414	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
MPJ80_RS01040 (208411)	208411..208704	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
MPJ80_RS01045 (208837)	208837..212022	-	3186	WP_001522867.1	ribonuclease E	-
MPJ80_RS01050 (212595)	212595..213554	+	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T238852 WP_000613626.1 NZ_CP093548:c208414-207920 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T238852 NZ_LR890508:2025724-2026002 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCATCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT238852 WP_001110447.1 NZ_CP093548:c208704-208411 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT238852 NZ_LR890508:2025461-2025721 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6