Detailed information of TA system TA238851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1359622..1360538
• Replicon: chromosome
• Accession: NZ_CP093546
• Organism: Bacillus sp. K1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: MOW04_RS07210
• Protein ID: WP_003244695.1
• Coordinates: 1359792..1360538 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: MOW04_RS07205
• Protein ID: WP_003232646.1
• Coordinates: 1359622..1359792 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOW04_RS07170	1356482..1356811	+	330	WP_046380959.1	XkdW family protein	-
MOW04_RS07175	1356808..1356972	+	165	WP_014479563.1	XkdX family protein	-
MOW04_RS07180	1357019..1357858	+	840	WP_046380960.1	phage-like element PBSX protein XepA	-
MOW04_RS07185	1357911..1358180	+	270	WP_015252265.1	hemolysin XhlA family protein	-
MOW04_RS07190	1358193..1358456	+	264	WP_003232653.1	phage holin	-
MOW04_RS07195	1358469..1359362	+	894	WP_003245230.1	N-acetylmuramoyl-L-alanine amidase	-
MOW04_RS07200	1359399..1359536	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
MOW04_RS07205	1359622..1359792	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
MOW04_RS07210	1359792..1360538	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
MOW04_RS07215	1360648..1361649	-	1002	WP_003232642.1	inorganic phosphate transporter	-
MOW04_RS07220	1361662..1362279	-	618	WP_003218470.1	DUF47 domain-containing protein	-
MOW04_RS07225	1362555..1363871	-	1317	WP_046380961.1	serine/threonine exchanger	-
MOW04_RS07230	1364260..1365210	+	951	WP_014479571.1	ring-cleaving dioxygenase	-
MOW04_RS07235	1365318..1365413	+	96	Protein_1362	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T238851 WP_003244695.1 NZ_CP093546:c1360538-1359792 [Bacillus sp. K1]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T238851 NZ_LR890508:c2019662-2019264 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT238851 WP_003232646.1 NZ_CP093546:c1359792-1359622 [Bacillus sp. K1]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT238851 NZ_LR890508:c2019958-2019665 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGTTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8