Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518989..519625 Replicon chromosome
Accession NZ_CP093546
Organism Bacillus sp. K1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag MOW04_RS02670 Protein ID WP_003156187.1
Coordinates 519275..519625 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag MOW04_RS02665 Protein ID WP_003225183.1
Coordinates 518989..519270 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOW04_RS02645 515349..515948 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
MOW04_RS02650 516043..516408 + 366 WP_015252768.1 holo-ACP synthase -
MOW04_RS02655 516574..517590 + 1017 WP_163131667.1 outer membrane lipoprotein carrier protein LolA -
MOW04_RS02660 517704..518873 + 1170 WP_163131601.1 alanine racemase -
MOW04_RS02665 518989..519270 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
MOW04_RS02670 519275..519625 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MOW04_RS02675 519740..520564 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
MOW04_RS02680 520569..520934 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
MOW04_RS02685 520938..521339 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
MOW04_RS02690 521351..522358 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
MOW04_RS02695 522420..522749 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
MOW04_RS02700 522746..523228 + 483 WP_003234299.1 anti-sigma B factor RsbW -
MOW04_RS02705 523194..523982 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
MOW04_RS02710 523982..524581 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T238850 WP_003156187.1 NZ_CP093546:519275-519625 [Bacillus sp. K1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T238850 NZ_LR890508:1791021-1791239 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT238850 WP_003225183.1 NZ_CP093546:518989-519270 [Bacillus sp. K1]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT238850 NZ_LR890508:1790621-1790995 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References