Detailed information of TA system TA023885

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3228932..3229595
• Replicon: chromosome
• Accession: NC_012779
• Organism: Edwardsiella ictaluri 93-146

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: C5BAS3
• Locus tag: NT01EI_RS14625
• Protein ID: WP_015872556.1
• Coordinates: 3228932..3229348 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: C5BAS4
• Locus tag: NT01EI_RS14630
• Protein ID: WP_015872557.1
• Coordinates: 3229329..3229595 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NT01EI_RS14605	3224856..3226589	-	1734	WP_015872552.1	single-stranded-DNA-specific exonuclease RecJ	-
NT01EI_RS14610	3226594..3227310	-	717	WP_015872553.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
NT01EI_RS14615	3227338..3228237	-	900	WP_015872554.1	site-specific tyrosine recombinase XerD	-
NT01EI_RS14620	3228395..3228907	+	513	WP_015872555.1	flavodoxin FldB	-
NT01EI_RS14625	3228932..3229348	-	417	WP_015872556.1	protein YgfX	Toxin
NT01EI_RS14630	3229329..3229595	-	267	WP_015872557.1	FAD assembly factor SdhE	Antitoxin
NT01EI_RS14635	3229943..3230938	+	996	WP_015872558.1	tRNA-modifying protein YgfZ	-
NT01EI_RS14640	3230979..3231803	-	825	WP_015872559.1	shikimate 5-dehydrogenase	-
NT01EI_RS14645	3231965..3232837	-	873	WP_015872560.1	nucleoside-specific channel-forming protein Tsx	-
NT01EI_RS14650	3233446..3234096	+	651	WP_035609190.1	hemolysin III family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16145.89 Da
• Isoelectric Point: 11.3233

>T23885 WP_015872556.1 NC_012779:c3229348-3228932 [Edwardsiella ictaluri 93-146]
VALWRCNVHASWRTQVMFLLGHGALVLAILLAPWPENYSVFWLILLVAVIFECIRSQRLIARNSGELQRLAPQVWHWQLR
EWRLARRPWVSDLGALLILQSTHGTPCRRRLWLAADSMSREEWGQLRRALLDTGDDRV

Nucleotide

• Length: 417 bp

>T23885 NC_012779:c3229348-3228932 [Edwardsiella ictaluri 93-146]
GTGGCCCTTTGGCGCTGTAATGTTCACGCCTCCTGGCGTACCCAGGTGATGTTTCTGCTGGGGCATGGCGCGCTGGTATT
GGCGATCCTGTTGGCGCCCTGGCCGGAAAACTACAGCGTATTCTGGCTGATTCTGCTGGTTGCGGTGATCTTTGAGTGTA
TCCGCAGCCAGCGTCTGATTGCGCGTAACAGCGGTGAGCTACAGCGGCTGGCCCCACAGGTATGGCACTGGCAGCTGCGC
GAGTGGCGCCTGGCCCGGCGCCCCTGGGTTAGCGATCTGGGAGCGTTGCTGATCCTGCAGAGTACCCATGGCACGCCGTG
CCGCCGTCGTCTGTGGCTGGCGGCCGACAGCATGAGCCGCGAAGAGTGGGGGCAGCTGCGCCGCGCGCTGCTCGATACGG
GCGACGATCGCGTATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10364.92 Da
• Isoelectric Point: 5.6951

>AT23885 WP_015872557.1 NC_012779:c3229595-3229329 [Edwardsiella ictaluri 93-146]
MDINNKARVHWACRRGMRELDIAIMPFFEHEYDALNDADKQTFVRLLSCDDPELFKWLMNQGKPDDTDLARMITLIQTRN
KERGPLAL

Nucleotide

• Length: 267 bp

>AT23885 NC_012779:c3229595-3229329 [Edwardsiella ictaluri 93-146]
ATGGATATCAATAATAAAGCGCGGGTTCACTGGGCGTGCCGCCGCGGAATGCGTGAGCTGGATATCGCCATTATGCCTTT
CTTTGAACATGAGTATGATGCGCTTAACGATGCCGATAAACAGACCTTTGTCCGACTGCTGTCCTGTGACGATCCTGAAC
TATTTAAATGGCTGATGAACCAGGGTAAGCCGGACGATACCGATCTGGCCCGTATGATTACGCTGATTCAGACGCGAAAT
AAAGAACGTGGCCCTTTGGCGCTGTAA

Structures

Toxin

• AlphaFold DB: C5BAS3

Antitoxin

• AlphaFold DB: C5BAS4