Detailed information of TA system TA238839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2170194..2171789
• Replicon: chromosome
• Accession: NZ_CP093535
• Organism: Ralstonia pseudosolanacearum strain SL1931

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: MNY32_RS09935
• Protein ID: WP_086706272.1
• Coordinates: 2170452..2171789 (+)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0S4UDK3
• Locus tag: MNY32_RS09930
• Protein ID: WP_016725279.1
• Coordinates: 2170194..2170448 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY32_RS09905 (MNY32_09905)	2166257..2167183	-	927	WP_016727232.1	muramoyltetrapeptide carboxypeptidase	-
MNY32_RS09910 (MNY32_09910)	2167180..2167731	-	552	WP_016727233.1	tRNA adenosine(34) deaminase TadA	-
MNY32_RS09915 (MNY32_09915)	2167751..2168227	-	477	WP_011001399.1	6-carboxytetrahydropterin synthase QueD	-
MNY32_RS09920 (MNY32_09920)	2168295..2168933	-	639	WP_020831956.1	7-carboxy-7-deazaguanine synthase	-
MNY32_RS09925 (MNY32_09925)	2169251..2170090	+	840	WP_016725278.1	oxaloacetate decarboxylase	-
MNY32_RS09930 (MNY32_09930)	2170194..2170448	+	255	WP_016725279.1	helix-turn-helix transcriptional regulator	Antitoxin
MNY32_RS09935 (MNY32_09935)	2170452..2171789	+	1338	WP_086706272.1	type II toxin-antitoxin system HipA family toxin	Toxin
MNY32_RS09940 (MNY32_09940)	2171786..2172988	+	1203	WP_011001394.1	MFS transporter	-
MNY32_RS09945 (MNY32_09945)	2173214..2173942	+	729	WP_021155798.1	DsbA family protein	-
MNY32_RS09950 (MNY32_09950)	2173955..2174551	-	597	WP_016727240.1	RNA 2',3'-cyclic phosphodiesterase	-
MNY32_RS09955 (MNY32_09955)	2174699..2175397	-	699	WP_016725453.1	protocatechuate 3,4-dioxygenase subunit alpha	-
MNY32_RS09960 (MNY32_09960)	2175394..2176134	-	741	WP_011001390.1	protocatechuate 3,4-dioxygenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-107)

Antitoxin

• Transcription regulator, HipB-like (10-66)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 48754.78 Da
• Isoelectric Point: 6.7232

>T238839 WP_086706272.1 NZ_CP093535:2170452-2171789 [Ralstonia pseudosolanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDGWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGTSKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Nucleotide

• Length: 1338 bp

>T238839 NZ_LR890502:c14275-13925 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 8754.07 Da
• Isoelectric Point: 10.4693

>AT238839 WP_016725279.1 NZ_CP093535:2170194-2170448 [Ralstonia pseudosolanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAGTPSAA
PADW

Nucleotide

• Length: 255 bp

>AT238839 NZ_LR890502:c13920-13618 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S4UDK3