Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 438876..439509 Replicon chromosome
Accession NZ_CP093535
Organism Ralstonia pseudosolanacearum strain SL1931

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag MNY32_RS01940 Protein ID WP_016725172.1
Coordinates 439096..439509 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag MNY32_RS01935 Protein ID WP_011002949.1
Coordinates 438876..439109 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY32_RS01920 (MNY32_01920) 434464..435759 - 1296 WP_011002952.1 MFS transporter -
MNY32_RS01925 (MNY32_01925) 435767..436999 - 1233 WP_225891636.1 AGE family epimerase/isomerase -
MNY32_RS01930 (MNY32_01930) 437212..438819 + 1608 WP_058908048.1 glucan biosynthesis protein -
MNY32_RS01935 (MNY32_01935) 438876..439109 + 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MNY32_RS01940 (MNY32_01940) 439096..439509 + 414 WP_016725172.1 PIN domain-containing protein Toxin
MNY32_RS01945 (MNY32_01945) 439484..440110 - 627 WP_058908047.1 DUF1415 domain-containing protein -
MNY32_RS01950 (MNY32_01950) 440301..440672 + 372 WP_011002946.1 hypothetical protein -
MNY32_RS01955 (MNY32_01955) 440733..442229 - 1497 WP_011002945.1 glycerol kinase GlpK -
MNY32_RS01960 (MNY32_01960) 442391..444127 - 1737 WP_058908046.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T238838 WP_016725172.1 NZ_CP093535:439096-439509 [Ralstonia pseudosolanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T238838 NZ_LR890501:c55378-54962 [Klebsiella pneumoniae]
GTGAACAAAATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATTACCTACGCCGAGATGCGCTTCGGCGCCACCGGGC
CGAAGGCCGCGCCGCGTCACGTCCAGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCATGGCCGGCACGCCGATTGGCCCGAACGACACGGCGATTGC
CGGACACGCCATCGCCGCCGGCGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTGCCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT238838 WP_011002949.1 NZ_CP093535:438876-439109 [Ralstonia pseudosolanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT238838 NZ_LR890501:c55605-55375 [Klebsiella pneumoniae]
ATGCGCACAGTCTCTATTTTTAAAAACGGCAACAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References