Detailed information of TA system TA238818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 589018..589547
• Replicon: chromosome
• Accession: NZ_CP093527
• Organism: Staphylococcus aureus strain 697R

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MPF55_RS02735
• Protein ID: WP_000621175.1
• Coordinates: 589185..589547 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MPF55_RS02730
• Protein ID: WP_000948331.1
• Coordinates: 589018..589188 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPF55_RS02700 (584054)	584054..584614	+	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-
MPF55_RS02705 (584823)	584823..585302	+	480	WP_001287083.1	hypothetical protein	-
MPF55_RS02710 (585295)	585295..586878	+	1584	WP_001294642.1	PH domain-containing protein	-
MPF55_RS02715 (586865)	586865..587356	+	492	WP_001205915.1	PH domain-containing protein	-
MPF55_RS02720 (587360)	587360..587719	+	360	WP_000581194.1	holo-ACP synthase	-
MPF55_RS02725 (587785)	587785..588933	+	1149	WP_001280702.1	alanine racemase	-
MPF55_RS02730 (589018)	589018..589188	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MPF55_RS02735 (589185)	589185..589547	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MPF55_RS02740 (589896)	589896..590897	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MPF55_RS02745 (591016)	591016..591342	+	327	WP_001052491.1	anti-sigma factor antagonist	-
MPF55_RS02750 (591344)	591344..591823	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
MPF55_RS02755 (591798)	591798..592568	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T238818 WP_000621175.1 NZ_CP093527:589185-589547 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T238818 NZ_LR890493:323051-323419 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT238818 WP_000948331.1 NZ_CP093527:589018-589188 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT238818 NZ_LR890493:322833-323054 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1