Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4158121..4158740 Replicon chromosome
Accession NZ_CP093501
Organism Klebsiella pneumoniae strain VA04

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MO409_RS20600 Protein ID WP_002892050.1
Coordinates 4158522..4158740 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MO409_RS20595 Protein ID WP_002892066.1
Coordinates 4158121..4158495 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO409_RS20585 (4153273) 4153273..4154466 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MO409_RS20590 (4154489) 4154489..4157635 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
MO409_RS20595 (4158121) 4158121..4158495 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MO409_RS20600 (4158522) 4158522..4158740 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MO409_RS20605 (4158899) 4158899..4159465 + 567 WP_002892030.1 maltose O-acetyltransferase -
MO409_RS20610 (4159437) 4159437..4159577 - 141 WP_004147370.1 hypothetical protein -
MO409_RS20615 (4159598) 4159598..4160068 + 471 WP_002892026.1 YlaC family protein -
MO409_RS20620 (4160043) 4160043..4161494 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
MO409_RS20625 (4161595) 4161595..4162293 + 699 WP_080899866.1 GNAT family protein -
MO409_RS20630 (4162290) 4162290..4162430 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MO409_RS20635 (4162430) 4162430..4162693 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T238806 WP_002892050.1 NZ_CP093501:4158522-4158740 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T238806 NZ_LR890485:55015-55497 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT238806 WP_002892066.1 NZ_CP093501:4158121-4158495 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT238806 NZ_LR890485:54761-55027 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References