Detailed information of TA system TA238793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 3519..3993
• Replicon: plasmid pVA32-7
• Accession: NZ_CP093500
• Organism: Klebsiella pneumoniae strain VA32

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: S1DZ70
• Locus tag: MO414_RS28370
• Protein ID: WP_000262326.1
• Coordinates: 3727..3993 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: MO414_RS28365
• Protein ID: WP_000879771.1
• Coordinates: 3519..3743 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MO414_RS28340 (MO414_28305)	146..280	-	135	WP_165451377.1	replication initiation protein	-
MO414_RS28345 (MO414_28310)	972..1292	+	321	WP_045265547.1	MobC family plasmid mobilization relaxosome protein	-
MO414_RS28350 (MO414_28315)	1501..1932	+	432	Protein_2	relaxase/mobilization nuclease domain-containing protein	-
MO414_RS28355 (MO414_28320)	1970..2455	+	486	WP_013631087.1	MbeB family mobilization protein	-
MO414_RS28360 (MO414_28325)	2459..2671	+	213	WP_000137423.1	MbeD family mobilization/exclusion protein	-
MO414_RS28365 (MO414_28330)	3519..3743	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
MO414_RS28370 (MO414_28335)	3727..3993	+	267	WP_000262326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MO414_RS28375 (MO414_28340)	4050..4295	-	246	WP_080090623.1	hypothetical protein	-
MO414_RS28380 (MO414_28345)	4622..6310	+	1689	WP_080090624.1	colicin-like bacteriocin tRNase domain-containing protein	-
MO414_RS28385 (MO414_28350)	6320..6577	+	258	WP_022644819.1	colicin E3-like toxin immunity protein	-
MO414_RS28390 (MO414_28355)	6662..6811	+	150	WP_080090625.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..6871	6871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10385.00 Da
• Isoelectric Point: 9.5211

>T238793 WP_000262326.1 NZ_CP093500:3727-3993 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRILCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T238793 NZ_LR890475:c256367-255882 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT238793 WP_000879771.1 NZ_CP093500:3519-3743 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT238793 NZ_LR890475:c256621-256355 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAGCTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCTCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: S1DZ70

Antitoxin

• AlphaFold DB: S1EJF5