Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 44711..45354 Replicon plasmid pVA32-105
Accession NZ_CP093497
Organism Klebsiella pneumoniae strain VA32

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag MO414_RS27215 Protein ID WP_001044770.1
Coordinates 44711..45127 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag MO414_RS27220 Protein ID WP_001261282.1
Coordinates 45124..45354 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO414_RS27195 (MO414_27155) 40080..40430 + 351 WP_004187110.1 DUF305 domain-containing protein -
MO414_RS27200 (MO414_27160) 40616..41524 - 909 WP_080933653.1 HNH endonuclease -
MO414_RS27205 (MO414_27165) 42068..43090 - 1023 WP_000361404.1 helicase UvrD -
MO414_RS27210 (MO414_27170) 43075..44637 - 1563 WP_004206609.1 AAA family ATPase -
MO414_RS27215 (MO414_27175) 44711..45127 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
MO414_RS27220 (MO414_27180) 45124..45354 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MO414_RS27225 (MO414_27185) 45929..46228 + 300 WP_052433691.1 hypothetical protein -
MO414_RS27230 (MO414_27190) 46283..46960 + 678 WP_064392774.1 hypothetical protein -
MO414_RS27235 (MO414_27195) 46957..47745 + 789 WP_064392776.1 site-specific integrase -
MO414_RS27240 (MO414_27200) 47790..48485 - 696 WP_040118530.1 replication initiation protein -
MO414_RS27245 (MO414_27205) 49096..49281 + 186 Protein_45 hypothetical protein -
MO414_RS27250 (MO414_27210) 49278..49811 - 534 Protein_46 Rpn family recombination-promoting nuclease/putative transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..104727 104727


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T238792 WP_001044770.1 NZ_CP093497:c45127-44711 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T238792 NZ_LR890475:210022-210327 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAAGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT238792 WP_001261282.1 NZ_CP093497:c45354-45124 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT238792 NZ_LR890475:209802-210020 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References