Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5208589..5209214 Replicon chromosome
Accession NZ_CP093495
Organism Klebsiella pneumoniae strain VA32

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MO414_RS25585 Protein ID WP_002882817.1
Coordinates 5208589..5208972 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MO414_RS25590 Protein ID WP_004150355.1
Coordinates 5208972..5209214 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO414_RS25570 (MO414_25535) 5205955..5206857 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MO414_RS25575 (MO414_25540) 5206854..5207489 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MO414_RS25580 (MO414_25545) 5207486..5208415 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MO414_RS25585 (MO414_25550) 5208589..5208972 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MO414_RS25590 (MO414_25555) 5208972..5209214 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MO414_RS25595 (MO414_25560) 5209419..5210336 + 918 WP_002882812.1 alpha/beta hydrolase -
MO414_RS25600 (MO414_25565) 5210350..5211291 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MO414_RS25605 (MO414_25570) 5211336..5211773 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MO414_RS25610 (MO414_25575) 5211770..5212630 - 861 WP_002882807.1 virulence factor BrkB family protein -
MO414_RS25615 (MO414_25580) 5212624..5213223 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T238790 WP_002882817.1 NZ_CP093495:c5208972-5208589 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T238790 NZ_LR890475:186690-187217 [Klebsiella pneumoniae]
GTGATGCAAGAGCTGGCGATAGAGATGATCAACGACGAATTTGACTATGACATAGGCGAATTCGATTGTGGAGAAGAAAC
CTTAAATACATTTTTGAAAGACCATCTCAAGCGGCAGCATGATGGGCAAATTTTAAAAGGTTATGTATTAGTAACCAAAG
AACAGAAGCCTAAGCTGTTAGGCTATTACACATTATCCGGGAGCTGTTTCGAGAGAAAAATGCTCCCGTCAAAAACACAG
CAAAGAAAAATCCCTTATCAGAATGCGCCAAGCATTACTTTGGGACGCTTGGCTATTGACAAATCAATTCAAGGTCAGGG
ATGGGGTGAATTGCTAGTGACTCACGCAATGAATGTCGTCTGGGATGCATCTAAGGCCGTAGGAATATATGGTCTCTTCG
TGGAAGCTCTGAACGATAAAGCTAAGCGCTTCTATACCAGATTAGATTTCATACAGCTCGTTGGTGAGAATAGTAATCTG
CTATTTTACCCGACAAAATCTATTGAAAAAATCTTTACCGAAGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT238790 WP_004150355.1 NZ_CP093495:c5209214-5208972 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT238790 NZ_LR890475:186413-186682 [Klebsiella pneumoniae]
ATGTCCGCACTGAGAAAGCAGCGCATAGATCTAAGATTAACTGATGACGACAAAAGCTTAATCGAAGAAGCCGCGGCAAT
GTCAAACCAGACTATTACACAGTTTGTAGTTGCCAGTGCCTCAGAACGTGCAGCGGAAGTGATTGAACAACATCGCAGAA
TGGTTCTAAACGAGGAGTCTTGGAACCTTGTCATGGATGCGATCGCAAACCCTGCTGCGCCGAATGAGCGGCTGAAGCGA
GCAGCGAAGCGTCTACAAAAAATGAGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References