Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4016626..4017245 Replicon chromosome
Accession NZ_CP093495
Organism Klebsiella pneumoniae strain VA32

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MO414_RS19855 Protein ID WP_002892050.1
Coordinates 4017027..4017245 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MO414_RS19850 Protein ID WP_002892066.1
Coordinates 4016626..4017000 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO414_RS19840 (MO414_19810) 4011778..4012971 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MO414_RS19845 (MO414_19815) 4012994..4016140 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
MO414_RS19850 (MO414_19820) 4016626..4017000 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MO414_RS19855 (MO414_19825) 4017027..4017245 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MO414_RS19860 (MO414_19830) 4017404..4017970 + 567 WP_002892030.1 maltose O-acetyltransferase -
MO414_RS19865 (MO414_19835) 4017942..4018082 - 141 WP_004147370.1 hypothetical protein -
MO414_RS19870 (MO414_19840) 4018103..4018573 + 471 WP_002892026.1 YlaC family protein -
MO414_RS19875 (MO414_19845) 4018548..4019999 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
MO414_RS19880 (MO414_19850) 4020100..4020798 + 699 WP_002892021.1 GNAT family protein -
MO414_RS19885 (MO414_19855) 4020795..4020935 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MO414_RS19890 (MO414_19860) 4020935..4021198 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T238786 WP_002892050.1 NZ_CP093495:4017027-4017245 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T238786 NZ_LR890474:c4569623-4569090 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT238786 WP_002892066.1 NZ_CP093495:4016626-4017000 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT238786 NZ_LR890474:c4569900-4569634 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References