Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5043081..5043706 Replicon chromosome
Accession NZ_CP093489
Organism Klebsiella pneumoniae strain VA126

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MO410_RS24575 Protein ID WP_002882817.1
Coordinates 5043081..5043464 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MO410_RS24580 Protein ID WP_004150355.1
Coordinates 5043464..5043706 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO410_RS24560 (5040447) 5040447..5041349 + 903 WP_004901686.1 formate dehydrogenase subunit beta -
MO410_RS24565 (5041346) 5041346..5041981 + 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
MO410_RS24570 (5041978) 5041978..5042907 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MO410_RS24575 (5043081) 5043081..5043464 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MO410_RS24580 (5043464) 5043464..5043706 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MO410_RS24585 (5043911) 5043911..5044828 + 918 WP_004901674.1 alpha/beta hydrolase -
MO410_RS24590 (5044842) 5044842..5045783 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MO410_RS24595 (5045828) 5045828..5046265 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MO410_RS24600 (5046262) 5046262..5047122 - 861 WP_002882807.1 virulence factor BrkB family protein -
MO410_RS24605 (5047116) 5047116..5047715 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T238772 WP_002882817.1 NZ_CP093489:c5043464-5043081 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T238772 NZ_LR890471:c3968517-3968200 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAAATGCGCAATGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT238772 WP_004150355.1 NZ_CP093489:c5043706-5043464 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT238772 NZ_LR890471:c3968207-3967920 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References