Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 53669..54312 Replicon plasmid pVA172-90
Accession NZ_CP093487
Organism Klebsiella pneumoniae strain VA172

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag MO413_RS25940 Protein ID WP_000754566.1
Coordinates 53896..54312 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag MO413_RS25935 Protein ID WP_001261276.1
Coordinates 53669..53899 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO413_RS25900 (MO413_25885) 48788..49057 + 270 WP_000339857.1 hypothetical protein -
MO413_RS25905 (MO413_25890) 49234..50100 - 867 WP_004118283.1 replication initiation protein -
MO413_RS25910 (MO413_25895) 50630..50734 + 105 WP_032409716.1 hypothetical protein -
MO413_RS25915 (MO413_25900) 50863..51120 + 258 WP_000764642.1 hypothetical protein -
MO413_RS25920 (MO413_25905) 51178..51954 - 777 WP_000015958.1 site-specific integrase -
MO413_RS25925 (MO413_25910) 51951..52694 - 744 WP_000129823.1 hypothetical protein -
MO413_RS25930 (MO413_25915) 52745..53095 - 351 WP_000493378.1 hypothetical protein -
MO413_RS25935 (MO413_25920) 53669..53899 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MO413_RS25940 (MO413_25925) 53896..54312 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
MO413_RS25945 (MO413_25930) 54516..55372 + 857 Protein_66 IS3-like element ISEc15 family transposase -
MO413_RS25950 (MO413_25935) 55654..55854 + 201 Protein_67 transposase -
MO413_RS25955 (MO413_25940) 55877..56299 + 423 WP_032446862.1 hypothetical protein -
MO413_RS25960 (MO413_25945) 56308..56805 + 498 WP_032446861.1 hypothetical protein -
MO413_RS25965 (MO413_25950) 56942..57277 - 336 Protein_70 Tn3 family transposase -
MO413_RS25970 (MO413_25955) 57797..57880 + 84 Protein_71 SOS response-associated peptidase -
MO413_RS25975 (MO413_25960) 57938..58009 - 72 Protein_72 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / blaCTX-M-2 / blaKPC-2 / ant(3'')-Ia - 1..90276 90276


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T238754 WP_000754566.1 NZ_CP093487:53896-54312 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T238754 NZ_LR890466:3548392-3548499 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT238754 WP_001261276.1 NZ_CP093487:53669-53899 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT238754 NZ_LR890466:c3548343-3548280 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References