Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5168508..5169133 Replicon chromosome
Accession NZ_CP093486
Organism Klebsiella pneumoniae strain VA172

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MO413_RS25250 Protein ID WP_002882817.1
Coordinates 5168508..5168891 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MO413_RS25255 Protein ID WP_004150355.1
Coordinates 5168891..5169133 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO413_RS25235 (5165874) 5165874..5166776 + 903 WP_004901686.1 formate dehydrogenase subunit beta -
MO413_RS25240 (5166773) 5166773..5167408 + 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
MO413_RS25245 (5167405) 5167405..5168334 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MO413_RS25250 (5168508) 5168508..5168891 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MO413_RS25255 (5168891) 5168891..5169133 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MO413_RS25260 (5169338) 5169338..5170255 + 918 WP_004901674.1 alpha/beta hydrolase -
MO413_RS25265 (5170269) 5170269..5171210 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MO413_RS25270 (5171255) 5171255..5171692 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MO413_RS25275 (5171689) 5171689..5172549 - 861 WP_002882807.1 virulence factor BrkB family protein -
MO413_RS25280 (5172543) 5172543..5173142 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T238753 WP_002882817.1 NZ_CP093486:c5168891-5168508 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T238753 NZ_LR890466:3547908-3548015 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT238753 WP_004150355.1 NZ_CP093486:c5169133-5168891 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT238753 NZ_LR890466:c3547850-3547796 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References