Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4557254..4558064 Replicon chromosome
Accession NZ_CP093486
Organism Klebsiella pneumoniae strain VA172

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag MO413_RS22380 Protein ID WP_004178461.1
Coordinates 4557254..4557787 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag MO413_RS22385 Protein ID WP_004222378.1
Coordinates 4557798..4558064 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO413_RS22375 (4556085) 4556085..4557206 + 1122 WP_110225560.1 cupin domain-containing protein -
MO413_RS22380 (4557254) 4557254..4557787 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
MO413_RS22385 (4557798) 4557798..4558064 - 267 WP_004222378.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MO413_RS22390 (4558167) 4558167..4559600 - 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
MO413_RS22395 (4559590) 4559590..4560273 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MO413_RS22400 (4560445) 4560445..4561830 + 1386 WP_004222371.1 efflux transporter outer membrane subunit -
MO413_RS22405 (4561848) 4561848..4562192 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T238749 WP_004178461.1 NZ_CP093486:c4557787-4557254 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T238749 NZ_LR890466:c2777946-2777773 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT238749 WP_004222378.1 NZ_CP093486:c4558064-4557798 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT238749 NZ_LR890466:c2778270-2777974 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References