Detailed information of TA system TA238735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 23123..23588
• Replicon: plasmid pVA564-33
• Accession: NZ_CP093485
• Organism: Klebsiella pneumoniae strain VA564

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: MO411_RS28140
• Protein ID: WP_001230707.1
• Coordinates: 23337..23588 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: MO411_RS28135
• Protein ID: WP_000801440.1
• Coordinates: 23123..23350 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MO411_RS28095 (MO411_28070)	18523..18756	-	234	WP_000121161.1	EexN family lipoprotein	-
MO411_RS28100 (MO411_28075)	18761..19027	-	267	WP_000160396.1	hypothetical protein	-
MO411_RS28105 (MO411_28080)	19104..20333	-	1230	WP_021544341.1	DNA relaxase/nickase TaxC	-
MO411_RS28110 (MO411_28085)	20337..20840	-	504	WP_141404785.1	DNA distortion polypeptide 1	-
MO411_RS28115 (MO411_28090)	21200..21292	-	93	WP_234631505.1	toxin-antitoxin system HicB family antitoxin	-
MO411_RS28120 (MO411_28095)	21636..21929	-	294	WP_000954638.1	hypothetical protein	-
MO411_RS28125 (MO411_28100)	21944..22318	-	375	WP_021544343.1	hypothetical protein	-
MO411_RS28130 (MO411_28105)	22370..22669	-	300	WP_000650311.1	hypothetical protein	-
MO411_RS28135 (MO411_28110)	23123..23350	-	228	WP_000801440.1	hypothetical protein	Antitoxin
MO411_RS28140 (MO411_28115)	23337..23588	-	252	WP_001230707.1	hypothetical protein	Toxin
MO411_RS28145 (MO411_28120)	23585..24127	-	543	WP_283557610.1	hypothetical protein	-
MO411_RS28150 (MO411_28125)	24232..24747	-	516	WP_001025395.1	J domain-containing protein	-
MO411_RS28155 (MO411_28130)	25387..25737	-	351	WP_000127482.1	addiction module antitoxin	-
MO411_RS28160 (MO411_28135)	25734..26069	-	336	WP_001147119.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MO411_RS28165 (MO411_28140)	27324..28190	+	867	WP_001353743.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33224	33224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T238735 WP_001230707.1 NZ_CP093485:c23588-23337 [Klebsiella pneumoniae]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T238735 NZ_LR890465:c158983-158540 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT238735 WP_000801440.1 NZ_CP093485:c23350-23123 [Klebsiella pneumoniae]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT238735 NZ_LR890465:c159210-158980 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9