Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5328541..5329166 Replicon chromosome
Accession NZ_CP093481
Organism Klebsiella pneumoniae strain VA564

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MO411_RS26070 Protein ID WP_002882817.1
Coordinates 5328541..5328924 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MO411_RS26075 Protein ID WP_004150355.1
Coordinates 5328924..5329166 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO411_RS26055 (5325907) 5325907..5326809 + 903 WP_004901686.1 formate dehydrogenase subunit beta -
MO411_RS26060 (5326806) 5326806..5327441 + 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
MO411_RS26065 (5327438) 5327438..5328367 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MO411_RS26070 (5328541) 5328541..5328924 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MO411_RS26075 (5328924) 5328924..5329166 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MO411_RS26080 (5329371) 5329371..5330288 + 918 WP_004901674.1 alpha/beta hydrolase -
MO411_RS26085 (5330302) 5330302..5331243 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MO411_RS26090 (5331288) 5331288..5331725 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MO411_RS26095 (5331722) 5331722..5332582 - 861 WP_002882807.1 virulence factor BrkB family protein -
MO411_RS26100 (5332576) 5332576..5333175 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T238733 WP_002882817.1 NZ_CP093481:c5328924-5328541 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T238733 NZ_LR890465:c59203-58892 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT238733 WP_004150355.1 NZ_CP093481:c5329166-5328924 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT238733 NZ_LR890465:c58895-58476 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References