Detailed information of TA system TA238714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 9711..10354
• Replicon: plasmid pVA569-35
• Accession: NZ_CP093478
• Organism: Klebsiella pneumoniae strain VA569

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: MO417_RS28290
• Protein ID: WP_000754566.1
• Coordinates: 9711..10127 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: MO417_RS28295
• Protein ID: WP_001261276.1
• Coordinates: 10124..10354 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MO417_RS28265 (MO417_28240)	6746..7081	+	336	Protein_4	Tn3 family transposase	-
MO417_RS28270 (MO417_28245)	7218..7715	-	498	WP_032446861.1	hypothetical protein	-
MO417_RS28275 (MO417_28250)	7724..8146	-	423	WP_032446862.1	hypothetical protein	-
MO417_RS28280 (MO417_28255)	8169..8369	-	201	Protein_7	transposase	-
MO417_RS28285 (MO417_28260)	8651..9507	-	857	Protein_8	IS3-like element ISEc15 family transposase	-
MO417_RS28290 (MO417_28265)	9711..10127	-	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
MO417_RS28295 (MO417_28270)	10124..10354	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MO417_RS28300 (MO417_28275)	10928..11278	+	351	WP_000493378.1	hypothetical protein	-
MO417_RS28305 (MO417_28280)	11329..12072	+	744	WP_000129823.1	hypothetical protein	-
MO417_RS28310 (MO417_28285)	12069..12845	+	777	WP_000015958.1	site-specific integrase	-
MO417_RS28315 (MO417_28290)	12903..13160	-	258	WP_000764642.1	hypothetical protein	-
MO417_RS28320 (MO417_28295)	13289..13393	-	105	WP_032409716.1	hypothetical protein	-
MO417_RS28325 (MO417_28300)	13923..14789	+	867	WP_004118283.1	replication initiation protein	-
MO417_RS28330 (MO417_28305)	14966..15235	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / qacE	-	1..35376	35376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T238714 WP_000754566.1 NZ_CP093478:c10127-9711 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T238714 NZ_LR890457:c4680007-4679675 [Klebsiella pneumoniae]
ATGAAAACTTTACCTGCAACAACTCCGCAGACGGCGAAGCTATGTCTGACGTCCGCGGATACCTGGCAAATGTTACTGAC
ACGTCTGCTAGAACAGCATTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCAGGGATCCCTCTGGCCGATGCAGTTAACTTTCTGGTGGATAAGTACGCACTGGTTCGTATCGATCGCAGAGGATTG
AGTTGGCAGGAGCAATCATCCTATCTCCGGCTTGTCGATACTCAGCGAACCATTAAGGTTATTGAGTTGTGGCTATTTGG
TGTAATGATGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT238714 WP_001261276.1 NZ_CP093478:c10354-10124 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT238714 NZ_LR890457:c4680345-4680028 [Klebsiella pneumoniae]
ATGAGCAACATCACTTGGGGCCTGCAGCGGGATATCACGCCGTGCCTGGGATCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGAGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGTTGGATGTCGTATTTCCAC
ATTTTATCAGTCAGATGGAATCGATGTTGACCACTGGTGAAATGAACCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTCCGGCTACGTATACATCGCTGTTTATCTCACCCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023