Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 72438..73081 Replicon plasmid pVA569-78
Accession NZ_CP093477
Organism Klebsiella pneumoniae strain VA569

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag MO417_RS28220 Protein ID WP_001034044.1
Coordinates 72665..73081 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag MO417_RS28215 Protein ID WP_001261286.1
Coordinates 72438..72668 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO417_RS28195 (69069) 69069..69824 - 756 WP_000852146.1 replication initiation protein RepE -
MO417_RS28200 (70546) 70546..71352 - 807 WP_000016970.1 site-specific integrase -
MO417_RS28205 (71353) 71353..71658 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
MO417_RS28210 (71660) 71660..71878 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
MO417_RS28215 (72438) 72438..72668 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MO417_RS28220 (72665) 72665..73081 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
MO417_RS28225 (73156) 73156..74721 + 1566 WP_001128474.1 AAA family ATPase -
MO417_RS28230 (74706) 74706..75728 + 1023 WP_000361402.1 helicase UvrD -
MO417_RS28240 (76745) 76745..77068 - 324 Protein_97 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA5 / aac(3)-IId / blaTEM-1B - 1..77813 77813


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T238713 WP_001034044.1 NZ_CP093477:72665-73081 [Klebsiella pneumoniae]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T238713 NZ_LR890457:c4640351-4639818 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATAAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT238713 WP_001261286.1 NZ_CP093477:72438-72668 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT238713 NZ_LR890457:c4640628-4640362 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References