Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 71353..71878 Replicon plasmid pVA569-78
Accession NZ_CP093477
Organism Klebsiella pneumoniae strain VA569

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MO417_RS28205 Protein ID WP_001159871.1
Coordinates 71353..71658 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag MO417_RS28210 Protein ID WP_000813630.1
Coordinates 71660..71878 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO417_RS28190 (67315) 67315..68481 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
MO417_RS28195 (69069) 69069..69824 - 756 WP_000852146.1 replication initiation protein RepE -
MO417_RS28200 (70546) 70546..71352 - 807 WP_000016970.1 site-specific integrase -
MO417_RS28205 (71353) 71353..71658 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MO417_RS28210 (71660) 71660..71878 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MO417_RS28215 (72438) 72438..72668 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
MO417_RS28220 (72665) 72665..73081 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
MO417_RS28225 (73156) 73156..74721 + 1566 WP_001128474.1 AAA family ATPase -
MO417_RS28230 (74706) 74706..75728 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA5 / aac(3)-IId / blaTEM-1B - 1..77813 77813


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T238712 WP_001159871.1 NZ_CP093477:c71658-71353 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T238712 NZ_LR890457:4040973-4041191 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATTCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT238712 WP_000813630.1 NZ_CP093477:c71878-71660 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT238712 NZ_LR890457:4040572-4040946 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References