Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5505447..5506072 Replicon chromosome
Accession NZ_CP093476
Organism Klebsiella pneumoniae strain VA569

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MO417_RS27380 Protein ID WP_002882817.1
Coordinates 5505447..5505830 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MO417_RS27385 Protein ID WP_004150355.1
Coordinates 5505830..5506072 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO417_RS27365 (MO417_27340) 5502813..5503715 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MO417_RS27370 (MO417_27345) 5503712..5504347 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MO417_RS27375 (MO417_27350) 5504344..5505273 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MO417_RS27380 (MO417_27355) 5505447..5505830 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MO417_RS27385 (MO417_27360) 5505830..5506072 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MO417_RS27390 (MO417_27365) 5506277..5507194 + 918 WP_002882812.1 alpha/beta hydrolase -
MO417_RS27395 (MO417_27370) 5507208..5508149 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MO417_RS27400 (MO417_27375) 5508194..5508631 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MO417_RS27405 (MO417_27380) 5508628..5509488 - 861 WP_002882807.1 virulence factor BrkB family protein -
MO417_RS27410 (MO417_27385) 5509482..5510081 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T238707 WP_002882817.1 NZ_CP093476:c5505830-5505447 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T238707 NZ_LR890457:386837-387184 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGACGATTACTATGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT238707 WP_004150355.1 NZ_CP093476:c5506072-5505830 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT238707 NZ_LR890457:387184-387483 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References