Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 85099..85835 Replicon plasmid pVA591-160
Accession NZ_CP093472
Organism Klebsiella pneumoniae strain VA591

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag MO415_RS26705 Protein ID WP_003026803.1
Coordinates 85099..85581 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MO415_RS26710 Protein ID WP_003026799.1
Coordinates 85569..85835 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO415_RS26680 (MO415_26650) 80261..80749 + 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -
MO415_RS26685 (MO415_26655) 80736..81698 + 963 WP_004152113.1 zinc metalloprotease HtpX -
MO415_RS26690 (MO415_26660) 81875..83040 + 1166 Protein_83 IS3 family transposase -
MO415_RS26695 83249..83380 - 132 WP_004218042.1 hypothetical protein -
MO415_RS26700 (MO415_26665) 83541..84887 - 1347 WP_020314316.1 ISNCY family transposase -
MO415_RS26705 (MO415_26670) 85099..85581 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
MO415_RS26710 (MO415_26675) 85569..85835 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MO415_RS26715 (MO415_26680) 86011..86265 - 255 WP_004152108.1 hypothetical protein -
MO415_RS26720 (MO415_26685) 86341..86598 - 258 WP_004152107.1 hypothetical protein -
MO415_RS26725 (MO415_26690) 86647..86850 - 204 WP_004152106.1 HHA domain-containing protein -
MO415_RS26730 (MO415_26695) 86884..87252 - 369 WP_004152105.1 hypothetical protein -
MO415_RS26735 (MO415_26700) 87296..87790 - 495 WP_004152104.1 DNA-binding protein -
MO415_RS26740 (MO415_26705) 87821..88396 - 576 WP_004152103.1 hypothetical protein -
MO415_RS26745 (MO415_26710) 88384..88653 - 270 WP_004152102.1 hypothetical protein -
MO415_RS26750 (MO415_26715) 89011..89361 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
MO415_RS26755 (MO415_26720) 89411..89773 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaSHV-26 / tet(D) - 1..160137 160137


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T238689 WP_003026803.1 NZ_CP093472:c85581-85099 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T238689 NZ_LR890446:c4665268-4664735 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT238689 WP_003026799.1 NZ_CP093472:c85835-85569 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT238689 NZ_LR890446:c4665545-4665279 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References