Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5352498..5353123 Replicon chromosome
Accession NZ_CP093471
Organism Klebsiella pneumoniae strain VA591

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MO415_RS25910 Protein ID WP_002882817.1
Coordinates 5352498..5352881 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MO415_RS25915 Protein ID WP_004150355.1
Coordinates 5352881..5353123 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO415_RS25895 (5349864) 5349864..5350766 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MO415_RS25900 (5350763) 5350763..5351398 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MO415_RS25905 (5351395) 5351395..5352324 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MO415_RS25910 (5352498) 5352498..5352881 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MO415_RS25915 (5352881) 5352881..5353123 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MO415_RS25920 (5353328) 5353328..5354245 + 918 WP_009484979.1 alpha/beta hydrolase -
MO415_RS25925 (5354259) 5354259..5355200 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MO415_RS25930 (5355245) 5355245..5355682 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MO415_RS25935 (5355679) 5355679..5356539 - 861 WP_004146232.1 virulence factor BrkB family protein -
MO415_RS25940 (5356533) 5356533..5357132 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T238688 WP_002882817.1 NZ_CP093471:c5352881-5352498 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T238688 NZ_LR890446:4041806-4042024 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT238688 WP_004150355.1 NZ_CP093471:c5353123-5352881 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT238688 NZ_LR890446:4041405-4041779 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References