Detailed information of TA system TA238683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3238275..3238906
• Replicon: chromosome
• Accession: NZ_CP093471
• Organism: Klebsiella pneumoniae strain VA591

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1Q8YTV3
• Locus tag: MO415_RS15875
• Protein ID: WP_012542177.1
• Coordinates: 3238730..3238906 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2X1QFN9
• Locus tag: MO415_RS15870
• Protein ID: WP_012542176.1
• Coordinates: 3238275..3238682 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MO415_RS15850 (3235346)	3235346..3235696	-	351	WP_017898986.1	hypothetical protein	-
MO415_RS15855 (3235693)	3235693..3236196	-	504	WP_275896059.1	lysozyme	-
MO415_RS15860 (3236168)	3236168..3236437	-	270	WP_032439907.1	phage holin	-
MO415_RS15865 (3236948)	3236948..3237994	-	1047	WP_283456762.1	IS481-like element ISKpn28 family transposase	-
MO415_RS15870 (3238275)	3238275..3238682	-	408	WP_012542176.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MO415_RS15875 (3238730)	3238730..3238906	-	177	WP_012542177.1	type II toxin-antitoxin system HicA family toxin	Toxin
MO415_RS15880 (3239271)	3239271..3240416	+	1146	WP_077271200.1	nucleoid-associated protein	-
MO415_RS15885 (3240406)	3240406..3241632	+	1227	WP_064147745.1	hypothetical protein	-
MO415_RS15890 (3241627)	3241627..3241971	-	345	WP_064147746.1	antiterminator Q family protein	-
MO415_RS15895 (3241984)	3241984..3243015	-	1032	WP_071010148.1	DUF968 domain-containing protein	-
MO415_RS15900 (3243215)	3243215..3243607	-	393	WP_023328106.1	hypothetical protein	-
MO415_RS15905 (3243648)	3243648..3243905	-	258	WP_022065893.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3204015..3257933	53918
	-	flank	IS/Tn	-	-	3236948..3237994	1046

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6632.83 Da
• Isoelectric Point: 11.0174

>T238683 WP_012542177.1 NZ_CP093471:c3238906-3238730 [Klebsiella pneumoniae]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Nucleotide

• Length: 177 bp

>T238683 NZ_LR890446:365627-365974 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14977.99 Da
• Isoelectric Point: 4.4277

>AT238683 WP_012542176.1 NZ_CP093471:c3238682-3238275 [Klebsiella pneumoniae]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARMINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Nucleotide

• Length: 408 bp

>AT238683 NZ_LR890446:365974-366273 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGGAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A1Q8YTV3

Antitoxin

• AlphaFold DB: A0A2X1QFN9