Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2925586..2926240 Replicon chromosome
Accession NC_012759
Organism Escherichia coli BW2952

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag BWG_RS14515 Protein ID WP_000244777.1
Coordinates 2925586..2925993 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BWG_RS14520 Protein ID WP_000354046.1
Coordinates 2925974..2926240 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWG_RS14495 (BWG_2617) 2921543..2923276 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
BWG_RS14500 (BWG_2618) 2923282..2923992 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BWG_RS14505 (BWG_2619) 2924017..2924913 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BWG_RS14510 (BWG_2620) 2925025..2925546 + 522 WP_001055874.1 flavodoxin FldB -
BWG_RS14515 (BWG_2621) 2925586..2925993 - 408 WP_000244777.1 toxin CptA Toxin
BWG_RS14520 (BWG_2622) 2925974..2926240 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BWG_RS14525 (BWG_2623) 2926483..2927463 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
BWG_RS14530 (BWG_2624) 2927659..2928318 - 660 WP_000250274.1 hemolysin III family protein -
BWG_RS14535 (BWG_2625) 2928482..2928793 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
BWG_RS14540 (BWG_2626) 2928838..2930271 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
BWG_RS14545 (BWG_2627) 2930328..2931071 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T23868 WP_000244777.1 NC_012759:c2925993-2925586 [Escherichia coli BW2952]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T23868 NC_012759:c2925993-2925586 [Escherichia coli BW2952]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT23868 WP_000354046.1 NC_012759:c2926240-2925974 [Escherichia coli BW2952]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT23868 NC_012759:c2926240-2925974 [Escherichia coli BW2952]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References