Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 325801..326387 Replicon chromosome
Accession NZ_CP093471
Organism Klebsiella pneumoniae strain VA591

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag MO415_RS01515 Protein ID WP_002920800.1
Coordinates 326019..326387 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag MO415_RS01510 Protein ID WP_004174006.1
Coordinates 325801..326022 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO415_RS01490 (321958) 321958..322884 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MO415_RS01495 (322881) 322881..324158 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
MO415_RS01500 (324155) 324155..324922 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MO415_RS01505 (324924) 324924..325637 + 714 WP_009484078.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MO415_RS01510 (325801) 325801..326022 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MO415_RS01515 (326019) 326019..326387 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MO415_RS01520 (326660) 326660..327976 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MO415_RS01525 (328083) 328083..328970 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MO415_RS01530 (328967) 328967..329812 + 846 WP_004174009.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MO415_RS01535 (329814) 329814..330884 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 315322..334412 19090


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T238672 WP_002920800.1 NZ_CP093471:326019-326387 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T238672 NZ_LR890434:1923394-1923496 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT238672 WP_004174006.1 NZ_CP093471:325801-326022 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT238672 NZ_LR890434:c1923503-1923358 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References