Detailed information of TA system TA238670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4869816..4870402
• Replicon: chromosome
• Accession: NZ_CP093465
• Organism: Klebsiella pneumoniae strain VA681

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: MO412_RS24010
• Protein ID: WP_002920800.1
• Coordinates: 4869816..4870184 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0H3GZM4
• Locus tag: MO412_RS24015
• Protein ID: WP_002920802.1
• Coordinates: 4870181..4870402 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MO412_RS23990 (MO412_23970)	4865319..4866389	-	1071	WP_002920787.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
MO412_RS23995 (MO412_23975)	4866391..4867236	-	846	WP_002920789.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
MO412_RS24000 (MO412_23980)	4867233..4868120	-	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
MO412_RS24005 (MO412_23985)	4868227..4869543	-	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
MO412_RS24010 (MO412_23990)	4869816..4870184	-	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MO412_RS24015 (MO412_23995)	4870181..4870402	-	222	WP_002920802.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MO412_RS24020 (MO412_24000)	4870566..4871279	-	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
MO412_RS24025 (MO412_24005)	4871281..4872048	-	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
MO412_RS24030 (MO412_24010)	4872045..4873322	-	1278	WP_002920806.1	branched chain amino acid ABC transporter permease LivM	-
MO412_RS24035 (MO412_24015)	4873319..4874245	-	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4864582..4875416	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T238670 WP_002920800.1 NZ_CP093465:c4870184-4869816 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T238670 NZ_LR890434:357574-357921 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8181.18 Da
• Isoelectric Point: 4.7769

>AT238670 WP_002920802.1 NZ_CP093465:c4870402-4870181 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT238670 NZ_LR890434:357921-358220 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A0H3GZM4