Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1215584..1216203 Replicon chromosome
Accession NZ_CP093465
Organism Klebsiella pneumoniae strain VA681

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MO412_RS05835 Protein ID WP_002892050.1
Coordinates 1215584..1215802 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MO412_RS05840 Protein ID WP_002892066.1
Coordinates 1215829..1216203 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO412_RS05800 (MO412_05800) 1211631..1211894 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
MO412_RS05805 (MO412_05805) 1211894..1212034 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MO412_RS05810 (MO412_05810) 1212031..1212729 - 699 WP_002892021.1 GNAT family protein -
MO412_RS05815 (MO412_05815) 1212830..1214281 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
MO412_RS05820 (MO412_05820) 1214256..1214726 - 471 WP_002892026.1 YlaC family protein -
MO412_RS05825 (MO412_05825) 1214747..1214887 + 141 WP_004147370.1 hypothetical protein -
MO412_RS05830 (MO412_05830) 1214859..1215425 - 567 WP_002892030.1 maltose O-acetyltransferase -
MO412_RS05835 (MO412_05835) 1215584..1215802 - 219 WP_002892050.1 HHA domain-containing protein Toxin
MO412_RS05840 (MO412_05840) 1215829..1216203 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MO412_RS05845 (MO412_05845) 1216689..1219835 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
MO412_RS05850 (MO412_05850) 1219858..1221051 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T238658 WP_002892050.1 NZ_CP093465:c1215802-1215584 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T238658 NZ_LR890426:c2800423-2800013 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATAGCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT238658 WP_002892066.1 NZ_CP093465:c1216203-1215829 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT238658 NZ_LR890426:c2800670-2800404 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References