Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 23382..24007 Replicon chromosome
Accession NZ_CP093465
Organism Klebsiella pneumoniae strain VA681

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MO412_RS00120 Protein ID WP_002882817.1
Coordinates 23624..24007 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MO412_RS00115 Protein ID WP_004150355.1
Coordinates 23382..23624 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO412_RS00090 (MO412_00090) 19373..19972 + 600 WP_004151865.1 glucose-1-phosphatase -
MO412_RS00095 (MO412_00095) 19966..20826 + 861 WP_002882807.1 virulence factor BrkB family protein -
MO412_RS00100 (MO412_00100) 20823..21260 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MO412_RS00105 (MO412_00105) 21305..22246 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MO412_RS00110 (MO412_00110) 22260..23177 - 918 WP_002882812.1 alpha/beta hydrolase -
MO412_RS00115 (MO412_00115) 23382..23624 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MO412_RS00120 (MO412_00120) 23624..24007 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MO412_RS00125 (MO412_00125) 24181..25110 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MO412_RS00130 (MO412_00130) 25107..25742 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MO412_RS00135 (MO412_00135) 25739..26641 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T238654 WP_002882817.1 NZ_CP093465:23624-24007 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T238654 NZ_LR890425:53397-53879 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT238654 WP_004150355.1 NZ_CP093465:23382-23624 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT238654 NZ_LR890425:53143-53409 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References